Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:210985
Wolfe AD, et al., Mixl1 localizes to putative axial stem cell reservoirs and their posterior descendants in the mouse embryo. Gene Expr Patterns. 2014 May;15(1):8-20
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6436901
|
Gene symbol:
|
Mixl1
|
Gene name:
|
Mix paired-like homeobox
|
|
Results
|
Specimen
1E:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
1E:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
1G:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
1G:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
1H:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
1H:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
2A:
embryonic day 7.0
(more )
Note: Mid-streak stage.
Specimen
2A:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Mid-streak stage.
|
Specimen
2B:
embryonic day 7.0
(more )
Note: Mid-streak stage.
Specimen
2B:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Mid-streak stage.
|
Specimen
2C:
embryonic day 7.0
(more )
Note: Late streak stage.
Specimen
2C:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Late streak stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: primitive streak |
Present |
|
2C
|
|
TS10: embryo mesoderm |
Present |
|
2C
|
Expression in presumptive laterally-migrating embryonic mesoderm.
|
TS10: embryo ectoderm |
Absent |
|
2C
|
No demonstrable expression in epiblast.
|
TS10: embryonic visceral endoderm |
Present |
Regionally restricted |
2C
|
Expression in embryo-associated visceral endoderm. Localization became biased toward posterior midline by late streak stage, with occasional expressing cells in anterior and lateral embryo-associated visceral endoderm.
|
Specimen
2D:
embryonic day 7.0
(more )
Note: Early bud stage.
Specimen
2D:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early bud stage.
|
Specimen
2E:
embryonic day 7.0
(more )
Note: Early bud stage.
Specimen
2E:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early bud stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: node |
Strong |
Regionally restricted |
2E
|
Strong expression in ventral node and posterior crown of Hensen's node.
|
TS10: posterior crown of node |
Strong |
Regionally restricted |
2E
|
Strong expression in ventral node and posterior crown of Hensen's node.
|
Specimen
2F:
embryonic day 7.0
(more )
Note: Early bud stage.
Specimen
2F:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early bud stage.
|
Specimen
2G:
embryonic day 7.0
(more )
Note: Early bud stage.
Specimen
2G:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early bud stage.
|
Specimen
2H:
embryonic day 7.5
(more )
Note: Late bud stage.
Specimen
2H:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Late bud stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: allantois |
Present |
Regionally restricted |
2H
|
Contiguous expression from primitive streak, extraembryonic primitive streak and allantoic bud.
|
TS11: primitive streak |
Present |
Regionally restricted |
2H
|
Contiguous expression from primitive streak, extraembryonic primitive streak and allantoic bud.
|
Specimen
2I:
embryonic day 7.5
(more )
Note: Late bud stage.
Specimen
2I:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Late bud stage.
|
Specimen
2J:
embryonic day 7.5
(more )
Note: Late bud stage.
Specimen
2J:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Late bud stage.
|
Specimen
3A:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3A:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
3B:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3B:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
LHF not shown:
embryonic day 7.5
(more )
Note: Late headfold stage.
Specimen
LHF not shown:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Late headfold stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: primitive streak |
Present |
Regionally restricted |
|
All streak-associated expression was ventrally localized except for posterior-most aspect at embryonic-extraembryonic junction where expression localized to full dorsal-ventral extent of streak.
|
Specimen
3C:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3C:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: primitive streak |
Present |
Regionally restricted |
3C
|
Expression most prominently localized to posterior primitive streak.
|
TS11: Allantoic Core Domain |
Strong |
Regionally restricted |
3C
|
Intense expression in ventral component of Allantoic Core Domain, adjacent to allantois-associated posterior visceral endoderm.
|
TS11: allantois-associated posterior visceral endoderm |
Weak |
Punctate |
3C
|
Authors describe intermittent, punctate, faintly expressing cells within allantois-associated posterior visceral endoderm.
|
TS11: allantois |
Weak |
Single cells |
3C
|
Punctate, faint expression in individual mesenchymal cells in distal allantois and overlying mesothelium.
|
Specimen
3G:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3G:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
3G inset:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3G inset:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
3J:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3J:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
3J inset:
embryonic day 7.5
(more )
Note: Early headfold stage.
Specimen
3J inset:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage.
|
Specimen
4A:
embryonic day 8.0
(more )
Note: 2 somite stage.
Specimen
4A:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
2 somite stage.
|
Specimen
4B:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
4B:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
4 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: embryo ectoderm |
Absent |
|
4B
|
No expression in epiblast.
|
TS12: embryo mesoderm |
Present |
Regionally restricted |
4B
|
No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
|
TS12: node |
Present |
Regionally restricted |
4B
|
No expression in node in this section.
|
Specimen
4C:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
4C:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
4 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: embryo ectoderm |
Absent |
|
4C
|
No expression in epiblast.
|
TS12: embryo mesoderm |
Present |
Regionally restricted |
4C
|
No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
|
TS12: posterior crown of node |
Present |
Clusters |
4C
|
Expression becomes evident and tightly clustered within posterior crown of the node.
|
Specimen
4D:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
4D:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
4 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: embryo ectoderm |
Absent |
|
4D
|
No expression in epiblast
|
TS12: embryo mesoderm |
Present |
Regionally restricted |
4D
|
No expression in embryonic mesoderm in this section but authors report expressng cells within mesoderm at midline posterior to node at each stage examined.
|
TS12: primitive streak |
Present |
Regionally restricted |
4D
|
No expression in primitive streak immediately posterior to posterior crown of node.
|
Specimen
4E:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
4E:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: posterior crown of node |
Present |
Regionally restricted |
4E
|
Expression at midline expanding posteriorly from posterior crown of node.
|
TS12: embryo mesoderm |
Present |
Scattered |
4E
|
Scattered expressing cells appearing within nascent embryonic mesoderm.
|
Specimen
5B:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
5B:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
16 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: tail bud |
Present |
Clusters |
5B
|
Expressing cells clustered within tailbud mesoderm adjacent to posterior end of hindgut.
|
TS14: notochord |
Present |
Regionally restricted |
5B
|
Expression localizes to putative posterior notochord population of axial mesodermal cells.
|
TS14: vitelline artery |
Absent |
|
5B
|
Expression excluded from omphalomesenteric artery.
|
TS14: primitive hindgut |
Present |
Regionally restricted |
5B
|
Expression excluded most of hindgut endoderm.
|
TS14: embryo mesoderm |
Present |
Scattered |
5B
|
Scattered expressing cells in mesoderm spanning notocord and expressing cells in tailgut mesoderm.
|
TS14: allantois |
Present |
Widespread |
5B
|
Expressing throughout allantois.
|
Specimen
5C:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
5C:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
16 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: embryo mesoderm |
Present |
Regionally restricted |
5C
|
Punctate expressing cells between tailbud mesoderm and notochord populations.
|
TS14: primitive hindgut |
Present |
Regionally restricted |
5C
|
Expression interspersed within dorsal hindgut endoderm.
|
TS14: surface ectoderm |
Present |
Single cells |
5C
|
Occasional expressing cell in embryonic surface ectoderm.
|
Specimen
5D:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
5D:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
16 somite stage.
|
Specimen
5E:
embryonic day 9.0
(more )
Note: 13 somite stage.
Specimen
5E:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
13 somite stage.
|
Specimen
5F:
embryonic day 9.0
(more )
Note: 13 somite stage.
Specimen
5F:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
13 somite stage.
|
Specimen
5G:
embryonic day 9.0
(more )
Note: 13 somite stage.
Specimen
5G:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
13 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: primitive hindgut |
Ambiguous |
Regionally restricted |
5G
|
Posterior to fusion of vitelline vessels, expression wanes within hindgut and omphalomesenteric artery. Authors state expression fades to undetectable.
|
TS14: vitelline artery |
Ambiguous |
Regionally restricted |
5G
|
Posterior to fusion of vitelline vessels, expression wanes within hindgut and omphalomesenteric artery. Authors state expression fades to undetectable.
|
Specimen
5H:
embryonic day 9.0
(more )
Note: 13 somite stage.
Specimen
5H:
(close )
Genetic Background:
|
involves: C57BL/6J * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
13 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: notochord |
Present |
Punctate |
5H
|
Limited punctate expressing cells in notochord.
|
TS14: yolk sac endoderm |
Weak |
|
5H
|
Relatively weak intensity of expression in visceral yolk sac endoderm.
|
Specimen
6A:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6A:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Specimen
6B:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6B:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: allantois |
Weak |
Regionally restricted |
6B
|
Faint expression throughout distal allantois and mesothelium.
|
Specimen
6C:
embryonic day 7.5; Runx1tm2Spe/Runx1 +
(more )
Note: Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6C:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Early headfold stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Specimen
6D:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6D:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: allantois |
Present |
Regionally restricted |
6D
|
Expression co-localizing with Runx1 within proximal allantois.
|
TS12: posterior crown of node |
Present |
Regionally restricted |
6D
|
Expression within presumptive posterior crown population.
|
Specimen
6E:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6E:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: umbilical blood vessel |
Strong |
Regionally restricted |
6E
|
In nascent umbilical vasculature in proximal allantois, strong expression in more proximal cells.
|
Specimen
6 somite stage not shown:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage.
Specimen
6 somite stage not shown:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: allantois |
Present |
Regionally restricted |
|
Co-expression with Runx1 in ventral allantoic mesothelium.
|
Specimen
6F:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6F:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive streak |
Strong |
Regionally restricted |
6F
|
Expression most intensely localized to primitive streak-associated cells adjacent to hindgut invagination.
|
TS12: embryo mesoderm |
Weak |
Regionally restricted |
6F
|
Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
|
TS12: primitive hindgut |
Weak |
Regionally restricted |
6F
|
Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
|
Specimen
6G:
embryonic day 8.0; Runx1tm2Spe/Runx1 +
(more )
Note: 6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6G:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
6 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: primitive streak |
Strong |
Regionally restricted |
6G
|
Expression most intensely localized to primitive streak-associated cells adjacent to hindgut invagination.
|
TS12: embryo mesoderm |
Weak |
Regionally restricted |
6G
|
Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
|
TS12: primitive hindgut |
Weak |
Regionally restricted |
6G
|
Faint colocalization with Runx1 reporter expression within ventral hindgut lip and proximo-ventral mesoderm.
|
Specimen
6H:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6H:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: primitive hindgut |
Present |
Regionally restricted |
6H
|
Expression in hindgut lip. Expression excluded from hindgut endoderm more posterior to hindgut lip.
|
TS13: blood island |
Present |
|
6H
|
|
TS13: vitelline artery |
Present |
Regionally restricted |
6H
|
Expression localized to developing blood cells of the omphalomesenteric artery.
|
Specimen
6I:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6I:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: yolk sac mesenchyme |
Present |
Widespread |
6I
|
Expression throughout yolk sac/blood island mesoderm.
|
TS13: extraembryonic endoderm |
Strong |
Regionally restricted |
6I
|
Expression strongly colocalizing with Runx1 reporter expression in extraembryonic visceral endoderm.
|
TS13: yolk sac |
Present |
Regionally restricted |
6I
|
Within visceral yolk sac, co-localization with Runx1 in both endothelium and blood cells. Stronger expression in endothelium.
|
TS13: blood island |
Present |
Regionally restricted |
6I
|
Expression colocalizes with Runx1 reporter expression in presumptive erythroblasts.
|
Specimen
6J:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 9 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6J:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
9 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: chorion ectoderm |
Present |
Regionally restricted |
6J
|
Expression in lateral chorionic ectoderm adjacent to site of contiguity with yolk sac. Co-expression to a lesser extent within lateral chorionic ectoderm.
|
TS13: extraembryonic endoderm |
Present |
Regionally restricted |
6J
|
Colocalization with Runx1 reporter expression within extraembryonic visceral endoderm contiguous with yolk sac.
|
Specimen
6K:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6K:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: neural tube |
Absent |
|
6K
|
No expression in neural tube or brain.
|
TS13: amnion |
Present |
Widespread |
6K
|
Expression throughout amnion and embryonic surface ectodderm.
|
TS13: surface ectoderm |
Present |
Widespread |
6K
|
Expression throughout amnion and embryonic surface ectodderm.
|
TS13: blood island |
Present |
Regionally restricted |
6K
|
Expression in yolk sac, blood islands and primitive erythroblasts.
|
TS13: yolk sac |
Present |
Regionally restricted |
6K
|
Expression in yolk sac, blood islands and primitive erythroblasts.
|
Specimen
6K inset:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6K inset:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
10 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: blood island |
Present |
Regionally restricted |
6K inset
|
Expression in some but not all circulating primitive erythroblasts within embryonic vasculature.
|
Specimen
6L:
embryonic day 8.5; Runx1tm2Spe/Runx1 +
(more )
Note: 9 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
Specimen
6L:
(close )
Genetic Background:
|
involves: 129S4/SvJae * C57BL/6J * CBA
|
Mutant Allele(s):
|
Runx1tm2Spe/Runx1+
|
Age:
|
embryonic day 8.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
9 somite stage. Double labeled: brown - Mixl1; blue - Runx1 reporter (assay MGI:6436904).
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: dorsal aorta |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
TS13: foregut diverticulum |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
TS13: heart |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
TS13: neural tube |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
TS13: notochord |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
TS13: paraxial mesenchyme |
Absent |
|
6L
|
Authors report no expression within any of the structures indicated in the figure.
|
|