Gene Expression Data
Assay Details
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Assay
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Reference:
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J:219234
Wang J, et al., FGF signaling is required for anterior but not posterior specification of the murine liver bud. Dev Dyn. 2015 Mar;244(3):431-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6446876
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Gene symbol:
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Fgf8
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Gene name:
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fibroblast growth factor 8
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Probe:
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Fgf8 cDNA13
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 8.0
(more  )
Note: 3-4 somite stage embryo, prior to liver bud induction
Specimen
1A:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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3-4 somite stage embryo, prior to liver bud induction
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: gut endoderm |
Present |
Regionally restricted |
1A
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Expression is seen in the prospective hepatic endoderm.
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| TS12: splanchnopleure |
Present |
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1A
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Expression is seen in the splanchnic mesoderm adjacent to the prospective hepatic primordium.
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Specimen
1B:
embryonic day 8.0
(more )
Note: 5-6 somite stage embryo, prior to liver bud induction
Specimen
1B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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5-6 somite stage embryo, prior to liver bud induction
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: splanchnopleure |
Present |
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1B
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Expression is seen in the splanchnic mesoderm adjacent to the prospective hepatic primordium; expression is no longer seen in the hepatic primordium.
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Specimen
1C:
embryonic day 8.0
(more )
Note: 7-8 somite stage embryo, liver bud induction initiated
Specimen
1C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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7-8 somite stage embryo, liver bud induction initiated
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: splanchnopleure |
Present |
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1C
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Expression is seen in the splanchnic mesoderm adjacent to the prospective hepatic primordium; expression is no longer seen in the hepatic primordium.
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Specimen
1D:
embryonic day 8.5
(more )
Note: 9-11 somite stage embryo, anterior liver bud formed
Specimen
1D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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9-11 somite stage embryo, anterior liver bud formed
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: splanchnopleure |
Present |
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1D
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Expression is seen in the splanchnic mesoderm adjacent to the hepatic primordium; expression is no longer seen in the hepatic primordium.
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Specimen
1E:
embryonic day 9.0
(more )
Note: 12-13 somite stage embryo, posterior liver bud formed
Specimen
1E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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12-13 somite stage embryo, posterior liver bud formed
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Analysis Tools