Ins2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:182781 Landsman L, et al., Pancreatic mesenchyme regulates epithelial organogenesis throughout development. PLoS Biol. 2011 Sep;9(9):e1001143
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6451467
Gene symbol:	Ins2
Gene name:	insulin II

Antibody:	anti-Insulin guinea pig
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Embryos were fixed in Z-fix (Anatech).

Results

7G ©

Specimen 7G: embryonic day 18.5 (more

)
Note: Littermate control. Triple labeled: green - Ins1 and Ins2; red - Gcg (assay MGI:6451468); blue - Sst (assay MGI:6451469).

Structure	Level	Pattern	Image	Note
TS26: endocrine pancreas	Present	Regionally restricted	7G	Expression is seen in beta cells.

7H ©

Specimen 7H: embryonic day 18.5; Ctnnb1^tm2Kem/Ctnnb1^tm2Kem, Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Ins1 and Ins2; red - Gcg (assay MGI:6451468); blue - Sst (assay MGI:6451469).

Structure	Level	Pattern	Image	Note
TS26: endocrine pancreas	Present	Regionally restricted	7H	The beta cell mass is reduced in mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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