Gene Expression Data
Assay Details
Assay
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Reference:
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J:136688
Buttler K, et al., Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristics. BMC Dev Biol. 2008;8:43
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6454672
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Gene symbol:
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Adgre1
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Gene name:
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adhesion G protein-coupled receptor E1
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Antibody:
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Anti F4/80 (BM8)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3:
embryonic day 13.5
(more )
Note: Double labeled: green - Adgre1; red - Lyve1 (assay MGI:6454344).
Specimen
3:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Adgre1; red - Lyve1 (assay MGI:6454344).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: dermatome |
Present |
Scattered |
3
|
A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
|
TS21: mediastinum |
Present |
Scattered |
3
|
A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
|
TS21: meninges |
Present |
Scattered |
3
|
A large number of scattered cells co-expressed Lyve1 and Adgre1, a macrophage marker. Some cells expressed only Lyve1 or Adgre1.
|
Specimen
4A:
embryonic day 11.5
(more )
Note: Double labeled: green - Adgre1; red - Prox1 (assay MGI:6454345).
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Adgre1; red - Prox1 (assay MGI:6454345).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: lymph sac |
Present |
|
4A
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Cells in the lymph sac expressed Adgre1 only or co-expressed Adgre1 and Prox1.
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Specimen
4B:
embryonic day 13.5
(more )
Note: Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: jugular lymph sac |
Present |
|
4F
,
4B
,
4C
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Cells were found in the developing lymph sacs that were positive for all three markers.
|
Specimen
5A:
embryonic day 13.5
(more )
Note: Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lymphatic vessel |
Present |
|
5A
|
In developing lymph vessels, a small number of cells were identified that were positive for all three markers. In very rare cases, various marker combinations were observed in adjacent cells of lymph vessels. e.g. 3 adjacent cells: one positive for Adgre1 only, one positive for Adgre1 and Lyve1, and a third positive for all three markers.
|
Specimen
5B:
embryonic day 13.5
(more )
Note: Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Adgre1; red - Prox1 (assay MGI:6454345); blue - Lyve1 (assay MGI:6454674).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: lymphatic vessel |
Present |
|
5B
|
In developing lymph vessels, a small number of cells were identified that were positive for all three markers.
|
|