Sftpc Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:265020 Tang X, et al., EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest. 2017 Dec 1;127(12):4314-4325
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6456988
Gene symbol:	Sftpc
Gene name:	surfactant associated protein C

Antibody:	anti-Pro-SP-C (WRAB-9337)
Assay notes:	Two proSP-C (Sftpc) antibodies were described; the one used in this assay was not detailed. Anti-proSP-C (MGI:6456917) may have been used in this assay.

Results

3C merge ©

3C proSP-C ©

Specimen 3C: embryonic day 18.5 (more

)
Note: Double labeled: green - proSP-C (Sftpc); red - mSP-C (Sftpc assay MGI:6456989).

Structure	Level	Pattern	Image	Note
TS26: lung	Present		3C merge , 3C proSP-C

3D merge ©

3D proSP-C ©

Specimen 3D: embryonic day 18.5; Emc3^{tm1c(EUCOMM)Wtsi}/Emc3^{tm1c(EUCOMM)Wtsi}, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: green - proSP-C (Sftpc); red - mSP-C (Sftpc assay MGI:6456989).

Structure	Level	Pattern	Image	Note
TS26: lung alveolus	Present	Regionally restricted	3D proSP-C , 3D merge	Expression accumulated in cytoplasm of alveolar type 2 (AT2) cells.

3E ©

Specimen 3E: embryonic day 18.5 (more

)
Note: Triple labeled: purple - proSP-C (Sftpc); green - proSP-B (Sftpb assay MGI:6456987); red - Lamp1 (assay MGI:6456990).

Structure	Level	Pattern	Image	Note
TS26: lung	Present		3E

3F ©

Specimen 3F: embryonic day 18.5; Emc3^{tm1c(EUCOMM)Wtsi}/Emc3^{tm1c(EUCOMM)Wtsi}, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Triple labeled: purple - proSP-C (Sftpc); green - proSP-B (Sftpb assay MGI:6456987); red - Lamp1 (assay MGI:6456990).

Structure	Level	Pattern	Image	Note
TS26: lung alveolus	Present	Regionally restricted	3F	Expression colocalized with LAMP-1 in alveolar type 2 (AT2) cells, indicating retention of unprocessed precursors.

S2E merge ©

S2E proSP-C ©

Specimen S2E: embryonic day 18.5 (more

)
Note: Double labeled: red - proSP-C (Sftpc); green - Hopx (assay MGI:6456993).

Structure	Level	Pattern	Image	Note
TS26: lung	Present		S2E proSP-C , S2E merge

S2F merge ©

S2F proSP-C ©

Specimen S2F: embryonic day 18.5; Emc3^{tm1c(EUCOMM)Wtsi}/Emc3^{tm1c(EUCOMM)Wtsi}, Shh^{tm1(EGFP/cre)Cjt}/Shh⁺ (more

)
Note: Conditional mutant. Double labeled: red - proSP-C (Sftpc); green - Hopx (assay MGI:6456993).

Structure	Level	Pattern	Image	Note
TS26: lung	Present		S2F proSP-C , S2F merge

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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