Antibody:	anti-fibronectin
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS15: tail mesenchyme	Present	Scattered	2C
TS15: neural fold	Present		2C	Expression is seen in the elevating neural folds of the posterior neuropore, in the basement membrane. It is less densely deposited near the midline.
TS15: neuropore	Present		2C	Expression is seen in the elevating neural folds of the posterior neuropore, in the basement membrane. It is less densely deposited near the midline.
TS15: blood vessel	Present		2C	Expression is associated with the basement membrane.
TS15: hindgut	Present		2C	Expression is associated with the basement membrane.
TS15: basement membrane	Present		2C

Structure	Level	Pattern	Image	Note
TS15: neural ectoderm	Strong	Regionally restricted	3C	A dense deposition is seen at the caudal extent of the neuroepithelium, the region where newly formed epithelium merges with neuroepithelium of the posterior neuropore.
TS15: neuropore	Strong	Regionally restricted	3C	A dense deposition is seen at the caudal extent of the neuroepithelium, the region where newly formed epithelium merges with neuroepithelium of the posterior neuropore.
TS15: hindgut	Present		3C	Staining is seen in the basement membrane.
TS15: notochord	Present		3C	Staining is seen in the basement membrane.
TS15: basement membrane	Present		3C
TS15: tail mesenchyme	Present		3C

Structure	Level	Pattern	Image	Note
TS16: tail mesenchyme	Present		4A
TS16: neural fold	Present		4A	The protein is present at the basal region of the radially organized cells; it is excluded from between the rosette of cells.

Structure	Level	Pattern	Image	Note
TS16: blood vessel	Present	Regionally restricted	4B	Staining is associated with the basement membrane.
TS16: hindgut	Present	Regionally restricted	4B	Staining is associated with the basement membrane.
TS16: neural tube	Present	Regionally restricted	4B	Staining is associated with the basement membrane.
TS16: notochord	Present	Regionally restricted	4B	Staining is associated with the basement membrane.
TS16: basement membrane	Present		4B