Gene Expression Data
Assay Details
Assay
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Reference:
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J:294771
Lantz B, et al., Finding the Unicorn, a New Mouse Model of Midfacial Clefting. Genes (Basel). 2020 Jan 11;11(1)
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6466999
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Gene symbol:
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Ezr
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Gene name:
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ezrin
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Probe:
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Erm1 probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The specificity of the Erm1 (zrin-radixin-moesin) probe used was not detailed; Rdx (MGI:97887) and Msn (MGI:97167) have also been annotated.
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Results
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Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: nasal septum mesenchyme |
Present |
Regionally restricted |
4C
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Expression in mesenchyme adjacent to Sox9-expressing region.
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Specimen
4G:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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b2b1941Clo/b2b1941Clo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: nasal mesenchyme |
Present |
Regionally restricted |
4G
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Expression in mesenchyme expanded superiorly between oral ectoderm and cartilaginous condensations.
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Specimen
7D:
embryonic day 11.5
(more )
Specimen
7D:
(close )
Genetic Background:
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involves: C57BL/6J
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7H:
(close )
Genetic Background:
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involves: C57BL/6J
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Mutant Allele(s):
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b2b1941Clo/b2b1941Clo
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: nasal process |
Present |
Regionally restricted |
7H
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Expression shifted cranially and laterally compared to control embryo.
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