Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:295480
Haage A, et al., Precise coordination of cell-ECM adhesion is essential for efficient melanoblast migration during development. Development. 2020 Jul 17;147(14):dev184234
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6467319
|
Gene symbol:
|
Dct
|
Gene name:
|
dopachrome tautomerase
|
|
Results
|
Specimen
1I Dorsal:
postnatal week 4
(more )
Note: Double labeled: green - Dct; white - DAPI.
Specimen
1I Dorsal:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dct; white - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair follicle |
Present |
Regionally restricted |
1I Dorsal
|
Authors report similar numbers of melanocytes per hair follicle in dorsal skin in wild-type and mutants.
|
Specimen
1J Dorsal:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dct; white - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair follicle |
Present |
Regionally restricted |
1J Dorsal
|
Authors report similar numbers of melanocytes per hair follicle in dorsal skin in wild-type and mutants.
|
Specimen
1I Ventral:
postnatal week 4
(more )
Note: Double labeled: green - Dct; white - DAPI.
Specimen
1I Ventral:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dct; white - DAPI.
|
Specimen
1J Ventral:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Dct; white - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: hair follicle |
Weak |
Regionally restricted |
1J Ventral
|
Decreased number of Dct-expressing melanocytes per hair follicle in ventral skin.
|
Specimen
1M:
postnatal week 4
(more )
Specimen
1M:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
1N:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
postnatal week 4
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3A:
embryonic day 15.5
(more )
Note: Specimens collected 4 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
Specimen
3A:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimens collected 4 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trunk skin |
Present |
|
3A (upper)
,
3A DCT
|
Authors report similar numbers of Dct and EdU double-labeled cells in mutant and control embryos at both time points.
|
Specimen
3B:
embryonic day 15.5; Tln1tm1.2Geno/ Tln1tm1.2Geno
(more )
Note: Specimens collected 4 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
Specimen
3B:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimens collected 4 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trunk skin |
Present |
|
3B (upper)
,
3B DCT
|
Authors report similar numbers of Dct and EdU double-labeled cells in mutant and control embryos at both time points.
|
Specimen
3C:
embryonic day 15.5
(more )
Note: Specimens collected 24 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
Specimen
3C:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimens collected 24 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: trunk skin |
Present |
|
3C (upper)
,
3C DCT
|
Authors report similar numbers of Dct and EdU double-labeled cells in mutant and control embryos at both time points.
|
Specimen
3D:
embryonic day 15.5; Tln1tm1.2Geno/ Tln1tm1.2Geno
(more )
Note: Specimens collected 24 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
Specimen
3D:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Specimens collected 24 hours following injection of EdU. Triple labeled: green - Dct; red - EdU; blue - DAPI.
|
Specimen
3F +/+:
embryonic day 15.5
(more )
Specimen
3F +/+:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3F E1770A/E1770A:
(close )
Genetic Background:
|
involves: C57BL/6J
|
Mutant Allele(s):
|
Tln1tm1.2Geno/Tln1tm1.2Geno
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|