Antibody:	anti-Tenascin C
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Widespread	1B E13.5 Middle	Widely expressed in palatal mesenchyme and underneath palatal epithelium.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Present	Widespread	1B E13.5 Mid-posterior	Widely expressed in palatal mesenchyme and underneath palatal epithelium.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf mesenchyme	Strong	Widespread	1B E13.5 Posterior	Stronger expression in posterior than middle or mid-posterior regions.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Widespread	1B E14.0 Middle	Widely expressed in palatal mesenchyme and underneath palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Widespread	1B E14.0 Mid-posterior	Widely expressed in palatal mesenchyme and underneath palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Strong	Widespread	1B E14.0 Posterior	Stronger expression in posterior than middle or mid-posterior regions.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Widespread	1B E14.5 Middle	Widely expressed in palatal mesenchyme and underneath the palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Present	Widespread	1B E14.5 Mid-posterior	Widely expressed in palatal mesenchyme and underneath the palatal epithelium.

Structure	Level	Pattern	Image	Note
TS22: palatal shelf mesenchyme	Very strong	Widespread	1B E14.5 Posterior	Stronger expression in posterior than middle or mid-posterior regions, particularly at later developmental stages.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Present	Widespread	1B E15.5 Middle	Widely expressed in palatal mesenchyme and underneath the palatal epithelium.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Present	Widespread	1B E15.5 Mid-posterior	Widely expressed in palatal mesenchyme and underneath the palatal epithelium.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf mesenchyme	Very strong	Widespread	1B E15.5 Posterior	Stronger expression in posterior than middle or mid-posterior regions, particularly at later developmental stages.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Present		6_E13.5 Control Middle

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Present		6_E13.5 Control Posterior

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Present	Regionally restricted	6_E13.5 Shh+/-;MFCS4+/- Middle	Expression detected in anterior region of palatal shelf.

Structure	Level	Pattern	Image	Note
TS21: palatal shelf	Weak	Regionally restricted	6_E13.5 Shh+/-;MFCS4+/- Posterior	Expression in posterior region slightly reduced at E13.5.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present		6_E15.0 Control Middle

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present		6_E15.0 Control Posterior

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Present	Regionally restricted	6_E15.0 Shh+/-;MFCS4+/- Middle	Expression detected in anterior region of palatal shelf.

Structure	Level	Pattern	Image	Note
TS23: palatal shelf	Absent		6_E15.0 Shh+/-;MFCS4+/- Posterior	Expression in posterior region completely lost at E15.0.