Gene Expression Data
Assay Details
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Assay
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Reference:
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J:294176
Snyder E, et al., ADAD1 and ADAD2, testis-specific adenosine deaminase domain-containing proteins, are required for male fertility. Sci Rep. 2020 Jul 14;10(1):11536
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6472838
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Gene symbol:
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Adad2
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Gene name:
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adenosine deaminase domain containing 2
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Antibody:
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ADAD2 antibody 1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Assay notes:
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Two antibodies against the carboxy terminus were generated and both generated the same results. The authors did not specify which was used, it is possible ADAD2 antibody 2 (MGI:6470841) was used.
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Results
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Specimen
1E:
postnatal adult
(more  )
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
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1E
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Expression is in pachytene spermatocytes.
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Specimen
1F IV:
postnatal adult
(more )
Specimen
1F IV:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1F IV
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Expression transitioned from diffusely cytoplasmic early in pachytene spermatocytes and coalesced into several perinuclear granules by late pachynema.
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| TS28: spermatid |
Present |
Regionally restricted |
1F IV
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Expression is dispersed early in round spermatids is undetectable in round spermatids by step 4.
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Specimen
1F VI:
postnatal adult
(more )
Specimen
1F VI:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1F VI
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Expression transitioned from diffusely cytoplasmic early in pachytene spermatocytes and coalesced into several perinuclear granules by late pachynema.
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| TS28: spermatid |
Present |
Regionally restricted |
1F VI
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Expression is dispersed early in round spermatids is undetectable in round spermatids by step 4.
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Specimen
1F VIII:
postnatal adult
(more )
Specimen
1F VIII:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1F VIII
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Expression transitioned from diffusely cytoplasmic early in pachytene spermatocytes and coalesced into several perinuclear granules by late pachynema.
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| TS28: spermatid |
Present |
Regionally restricted |
1F VIII
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Expression is dispersed early in round spermatids is undetectable in round spermatids by step 4.
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Specimen
1F X:
postnatal adult
(more )
Specimen
1F X:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1F X
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Expression transitioned from diffusely cytoplasmic early in pachytene spermatocytes and coalesced into several perinuclear granules by late pachynema.
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| TS28: spermatid |
Present |
Regionally restricted |
1F X
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Expression is dispersed early in round spermatids is undetectable in round spermatids by step 4.
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Specimen
1F II-III:
postnatal adult
(more )
Specimen
1F II-III:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatocyte |
Present |
Regionally restricted |
1F II-III
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Expression transitioned from diffusely cytoplasmic early in pachytene spermatocytes and coalesced into several perinuclear granules by late pachynema.
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| TS28: spermatid |
Present |
Regionally restricted |
1F II-III
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Expression is dispersed early in round spermatids is undetectable in round spermatids by step 4.
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Specimen
2E Wildtype:
postnatal adult
(more )
Specimen
2E Wildtype:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2E Ada2:
(close )
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Genetic Background:
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involves: C57BL/6NJ
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Mutant Allele(s):
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Adad2em3(IMPC)J/Adad2em3(IMPC)J
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
postnatal adult
(more )
Note: Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
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Specimen
5C Stage VII:
postnatal adult
(more )
Note: Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
Specimen
5C Stage VII:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
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Specimen
5C Stage IX:
postnatal adult
(more )
Note: Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
Specimen
5C Stage IX:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
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Specimen
5C Stage XI:
postnatal adult
(more )
Note: Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
Specimen
5C Stage XI:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Male
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Adad2; red - Ddx25 (assay MGI:6472899).
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Analysis Tools