Antibody:	anti-Calbindin D28k (CB-38), rabbit
Assay notes:	Non-homozygous mutant animals were either wildtype or heterozygous.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		2L

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2M

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2N

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2O

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		2L inset

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2M inset

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2N inset

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2O inset

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		3A

Structure	Level	Pattern	Image	Note
TS26: cerebellum Purkinje cell layer	Present		3B	Distribution of Purkinje cells similar to that of control.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3E	Purkinje cells in 2-3 cell-thick well-organized multilayer beneath external granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3F	Purkinje cells in 2-3 cell-thick well-organized multilayer beneath external granular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3G	Purkinje cell distribution similar to control, abut at some locations less well organized and forming a 4-5 cell-thick multilayer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3H	Purkinje cell distribution similar to control, abut at some locations less well organized and forming a 4-5 cell-thick multilayer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3K	Purkinje cells completing reorganization as a regular monolayer with cell bodies aligned abd dendritic arbors regularly oriented toward molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3L	Purkinje cells completing reorganization as a regular monolayer with cell bodies aligned abd dendritic arbors regularly oriented toward molecular layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3M	Alignment of Purkinje cells less regular than in control, and orientation of dendritic arbor severely perturbed.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	3N	In some regions, Purkinje cells remain as a 2 cell-thick multilayer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4C	Purkinje cells organized as continuous monolayer with regular spaced cell bodies and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4D	Purkinje cells organized as continuous monolayer with regular spaced cell bodies and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4E	Purkinje cells organized as continuous monolayer with regular spaced cell bodies and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4F	Purkinje cells organized as continuous monolayer with regular spaced cell bodies and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4G	In some locations, Purkinje cells organized as monolayer with regular and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4H	Authors report Purkinje cells were absent at some locations with cell layer discontinuous in some regions.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4I	Authors report Purkinje cells were absent at some locations with cell layer discontinuous in some regions.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted	4J	Purkinje cells not properly aligned in monolayer and abnormally located in granule cell layer.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4M	Purkinje cells organized as monolayer with regular and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4N	Purkinje cells organized as monolayer with regular and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4O	Purkinje cells organized as monolayer with regular and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4P	Purkinje cells organized as monolayer with regular and aligned arrangement.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4Q	Purkinje cells organized as monolayer with regular and aligned arrangement in some locations.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4R	In some locations, Purkinje cells were absent.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4S	At some locations, Purkinje cells were not properly aligned in monolayer - 2-3 Purkinje cells found superposed.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	4T	In some locations, Purkinje cells were absent.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4G'

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		4H'

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4I'

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4J'

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4K'

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4L'

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5G

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5H

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5I

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5J

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		5K	Purkinje cell disposition in some regions was similar to control mice.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5L	At some locations, Purkinje cells were superimposed.

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	5M	At some locations, Purkinje cells were absent.

Structure	Level	Pattern	Image	Note
TS28: cerebellum granule cell layer	Present	Regionally restricted	5N	At some locations, Purkinje cells were abnormally located in granule cells layer.