Kit Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:300507 Gao P, et al., Transcriptional regulatory network controlling the ontogeny of hematopoietic stem cells. Genes Dev. 2020 Jul 1;34(13-14):950-964
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6511250
Gene symbol:	Kit
Gene name:	KIT proto-oncogene receptor tyrosine kinase

Antibody:	anti-CD117 (2B8)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

7Ai +/+ ©

7Aiii +/+ ©

Specimen 7A +/+: embryonic day 10.5 (more

)
Note: Triple labeled: white - Kit; magenta - Pecam1 (CD31; assay MGI:6511248); teal - Runx1 (assay MGI:6511249)

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	7Ai +/+ , 7Aiii +/+	Expression is seen in intra-arterial hematopoietic clusters.

7Ai -/- ©

7Aiii -/- ©

Specimen 7A -/-: embryonic day 10.5; Sp3^tm1Sus/Sp3^tm1Sus (more

)
Note: Triple labeled: white - Kit; magenta - Pecam1 (CD31; assay MGI:6511248); teal - Runx1 (assay MGI:6511249)

Structure	Level	Pattern	Image	Note
TS17: hematopoietic system	Present	Clusters	7Aiii -/- , 7Ai -/-	Expression is seen in intra-arterial hematopoietic clusters; their number is reduced in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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