Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:297547
Leca I, et al., A proteomic survey of microtubule-associated proteins in a R402H TUBA1A mutant mouse. PLoS Genet. 2020 Nov;16(11):e1009104
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6695138
|
|
Gene symbol:
|
Rbfox3
|
|
Gene name:
|
RNA binding protein, fox-1 homolog (C. elegans) 3
|
|
Antibody:
|
Anti-NeuN (clone A60; MAB377)
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor
|
|
Assay notes:
|
Specimens were subjected to heat-mediated antigen retrieval.
|
|
|
Results
|
Specimen
1F:
postnatal week 8-10; Emx1tm1(cre)Krj/Emx1 +
(more  )
Note: coronal section
Specimen
1F:
(close  )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Emx1tm1(cre)Krj/Emx1+
|
|
Age:
|
postnatal week 8-10
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
coronal section
|
Specimen
1G:
postnatal week 8-10; Tuba1atm1.1Dak/Tuba1a +
(more )
Note: coronal section
Specimen
1G:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Tuba1atm1.1Dak/Tuba1a+
|
|
Age:
|
postnatal week 8-10
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
coronal section
|
Specimen
1H:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6J
|
|
Mutant Allele(s):
|
Tuba1atm1.1Dak/Tuba1a+
Emx1tm1(cre)Krj/Emx1+
|
|
Age:
|
postnatal week 8-10
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Conditional mutant. coronal section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Widespread |
1H
|
Expression is present in neurons. There is no significant difference in cortical thickness in the mutant compared to the controls.
|
|
Analysis Tools