Sycp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6695313
Gene symbol:	Sycp3
Gene name:	synaptonemal complex protein 3

Antibody:	Anti-Sycp3 (NB300-232)
Assay notes:	Two antibodies were described MGI:5805440 and MGI:6694240; either could have been used in this assay.

Results

1d merge ©

1d Sycp3 ©

Specimen 1d: postnatal adult (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		1d merge , 1d Sycp3

1e merge ©

1e Sycp3 ©

Specimen 1e: embryonic day 15.5 (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: ovary	Present		1e merge , 1e Sycp3

2b Prelepto merge ©

2b Prelepto Sycp3 ©

Specimen 2b Prelepto: postnatal (more

)
Note: Preleptotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Prelepto Sycp3 , 2b Prelepto merge

2b Lepto merge ©

2b Lepto Sycp3 ©

Specimen 2b Lepto: postnatal (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Lepto merge , 2b Lepto Sycp3

2b Zygo merge ©

2b Zygo Sycp3 ©

Specimen 2b Zygo: postnatal (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Zygo merge , 2b Zygo Sycp3

2b Pachy merge ©

2b Pachy Sycp3 ©

Specimen 2b Pachy: postnatal (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Pachy merge , 2b Pachy Sycp3

2b Diplo merge ©

2b Diplo Sycp3 ©

Specimen 2b Diplo: postnatal (more

)
Note: Diplotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Diplo merge , 2b Diplo Sycp3

2b Zygo-like merge ©

2b Zygo-like Sycp3 ©

Specimen 2b Zygo-like: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		2b Zygo-like Sycp3 , 2b Zygo-like merge

4c Meioc+/+ Sycp3/Dapi ©

Specimen 4c Meioc+/+: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		4c Meioc+/+ Sycp3/Dapi

4c Meioc-/- Sycp3/Dapi ©

Specimen 4c Meioc-/-: embryonic day 15.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Weak		4c Meioc-/- Sycp3/Dapi	The meiotic marker was poorly detected, although germ cell density appeared unaltered.

5d 8dpp Meioc+/+ ©

Specimen 5d 8-dpp Meioc+/+: postnatal day 8 (more

)
Note: Double labeled: red - Sycp3; green - Stra8 (assay MGI:6695333).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5d 8dpp Meioc+/+

5d 8dpp Meioc-/- ©

Specimen 5d 8-dpp Meioc-/-: postnatal day 8; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Stra8 (assay MGI:6695333).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Weak		5d 8dpp Meioc-/-

5d 16dpp Meioc+/+ ©

Specimen 5d 16-dpp Meioc+/+: postnatal day 16 (more

)
Note: Double labeled: red - Sycp3; green - Stra8 (assay MGI:6695333).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5d 16dpp Meioc+/+

5d 16dpp Meioc-/- ©

Specimen 5d 16-dpp Meioc-/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Stra8 (assay MGI:6695333).

Structure	Level	Pattern	Image	Note
TS28: germ cell of testis	Present		5d 16dpp Meioc-/-

6a Meioc+/+ Lepto Sycp1/Sycp3 ©

Specimen 6a Lepto Meioc+/+: postnatal day 16 (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc+/+ Lepto Sycp1/Sycp3

6a Meioc-/- Lepto Sycp1/Sycp3 ©

Specimen 6a Lepto Meioc-/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc-/- Lepto Sycp1/Sycp3	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6a Meioc+/+ Zygo Sycp1/Sycp3 ©

Specimen 6a Zygo Meioc+/+: postnatal day 16 (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc+/+ Zygo Sycp1/Sycp3

6a Meioc-/- Zygo-like Sycp1/Sycp3 top ©

Specimen 6a Zygo-like Meioc-/- top: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc-/- Zygo-like Sycp1/Sycp3 top	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6a Meioc+/+ Pachy Sycp1/Sycp3 ©

Specimen 6a Pachy Meioc+/+: postnatal day 16 (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc+/+ Pachy Sycp1/Sycp3

6a Meioc-/- Zygo-like Sycp1/Sycp3 bottom ©

Specimen 6a Zygo-like Meioc-/- bottom: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Sycp1 (assay MGI:6715977).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6a Meioc-/- Zygo-like Sycp1/Sycp3 bottom	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6b Meioc+/+ Lepto Sycp3 ©

6b Meioc+/+ Lepto Sycp3/gamma-H2ax ©

Specimen 6b Lepto Meioc+/+: postnatal day 16 (more

)
Note: Leptotene. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc+/+ Lepto Sycp3 , 6b Meioc+/+ Lepto Sycp3/gamma-H2ax

6b Meioc-/- Lepto Sycp3 ©

6b Meioc-/- Lepto Sycp3/gamma-H2ax ©

Specimen 6b Lepto Meioc-/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc-/- Lepto Sycp3 , 6b Meioc-/- Lepto Sycp3/gamma-H2ax	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6b Meioc+/+ Zygo Sycp3 ©

6b Meioc+/+ Zygo Sycp3/gamma-H2ax ©

Specimen 6b Zygo Meioc+/+: postnatal day 16 (more

)
Note: Zygotene. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc+/+ Zygo Sycp3 , 6b Meioc+/+ Zygo Sycp3/gamma-H2ax

6b Meioc-/- Zygo-like Sycp3 top ©

6b Meioc-/- Zygo-like Sycp3/gamma-H2ax top ©

Specimen 6b Zygo-like Meioc-/- top: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc-/- Zygo-like Sycp3/gamma-H2ax top , 6b Meioc-/- Zygo-like Sycp3 top	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6b Meioc+/+ Pachy Sycp3 ©

6b Meioc+/+ Pachy Sycp3/gamma-H2ax ©

Specimen 6b Pachy Meioc+/+: postnatal day 16 (more

)
Note: Pachytene. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc+/+ Pachy Sycp3 , 6b Meioc+/+ Pachy Sycp3/gamma-H2ax

6b Meioc-/- Zygo-like Sycp3 bottom ©

6b Meioc-/- Zygo-like Sycp3/gamma-H2ax bottom ©

Specimen 6b Zygo-like Meioc-/- bottom: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: green - Sycp3; red - H2ax (assay MGI:6715978).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6b Meioc-/- Zygo-like Sycp3 bottom , 6b Meioc-/- Zygo-like Sycp3/gamma-H2ax bottom	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

6c Meioc+/+ Pachy ©

Specimen 6c Meioc+/+: postnatal day 16 (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Kash5 (assay MGI:6715979).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6c Meioc+/+ Pachy

6c Meioc-/- Zygo-like ©

Specimen 6c Meioc-/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Kash5 (assay MGI:6715979).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		6c Meioc-/- Zygo-like	Most mutant spermatocytes were similar to preleptotene and leptotene stages with partial and discontinuous Sycp3 staining along the chromosome axes; occasionally, cells progressed further to display continuous Sycp3.

9d top left ©

Specimen 9d Meioc+/+ 8-dpp: postnatal day 8 (more

)
Note: Double labeled: red - Sycp3; green - Ythdc2 (assay MGI:6716019).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		9d top left

9d bottom left ©

Specimen 9d Meioc-/- 8-dpp: postnatal day 8; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Ythdc2 (assay MGI:6716019).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		9d bottom left

9d top right ©

Specimen 9d Meioc+/+ 16-dpp: postnatal day 16 (more

)
Note: Double labeled: red - Sycp3; green - Ythdc2 (assay MGI:6716019).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		9d top right

9d bottom right ©

Specimen 9d Meioc-/- 16-dpp: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Ythdc2 (assay MGI:6716019).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		9d bottom right

S3b left ©

Specimen S3b left: postnatal day 16 (more

)

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S3b left

S3b right ©

Specimen S3b right: postnatal day 16 (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S3b right

S3c Meioc+/+ ©

Specimen S3c Meioc+/+: embryonic day 15.5 (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Meioc+/+

S3c Meioc-/- ©

Specimen S3c Meioc-/-: embryonic day 15.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Meioc-/-

S3c Lepto ©

Specimen S3c Meioc+/+ Lepto: embryonic day 15.5 (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Lepto

S3c Zygo ©

Specimen S3c Meioc+/+ Zygo: embryonic day 15.5 (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Zygo

S3c Pachy ©

Specimen S3c Meioc+/+ Pachy: embryonic day 15.5 (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Pachy

S3c Diplo ©

Specimen S3c Meioc+/+ Diplo: embryonic day 18.5 (more

)
Note: Diplotene. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Present		S3c Diplo

S3c Lepto-like ©

Specimen S3c Meioc-/- Lepto-like: embryonic day 15.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene-like. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS23: meiotic germ cell of ovary	Present		S3c Lepto-like

S3c Zygo-like ©

Specimen S3c Meioc-/- Zygo-like: embryonic day 18.5; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS26: meiotic germ cell of ovary	Present		S3c Zygo-like

S3d Meioc+/+ ©

Specimen S3d Meioc+/+: postnatal adult (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S3d Meioc+/+

S3d Meioc-/- ©

Specimen S3d Meioc-/-: postnatal adult; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Meioc (assay MGI:6695312).

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S3d Meioc-/-

S5a Meioc+/+ left ©

S5a Meioc+/+ right ©

Specimen s5a Meioc+/+: postnatal (more

)
Note: Double labeled: red - Sycp3; green - Rec8 (assay MGI:6716277).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5a Meioc+/+ right , S5a Meioc+/+ left

S5a Meioc-/- left ©

S5a Meioc-/- right ©

Specimen s5a Meioc-/-: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Rec8 (assay MGI:6716277).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5a Meioc-/- right , S5a Meioc-/- left

S5b Meioc+/+ left ©

S5b Meioc+/+ right ©

Specimen s5b Meioc+/+: postnatal (more

)
Note: Double labeled: red - Sycp3; green - Smc3 (assay MGI:6716278).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5b Meioc+/+ left , S5b Meioc+/+ right

S5b Meioc-/- left ©

S5b Meioc-/- right ©

Specimen s5b Meioc-/-: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - Smc3 (assay MGI:6716278).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5b Meioc-/- right , S5b Meioc-/- left

S5c Meioc+/+ Lepto ©

Specimen s5c Meioc+/+ Lepto: postnatal (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc+/+ Lepto

S5c Meioc-/- Lepto ©

Specimen s5c Meioc-/- Lepto: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc-/- Lepto

S5c Meioc+/+ Zygo ©

Specimen s5c Meioc+/+ Zygo: postnatal (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc+/+ Zygo

S5c Meioc-/- Zygo-like left ©

Specimen s5c Meioc-/- Zygo-like left: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc-/- Zygo-like left

S5c Meioc+/+ Pachy ©

Specimen s5c Meioc+/+ Pachy: postnatal (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc+/+ Pachy

S5c Meioc-/- Zygo-like right ©

Specimen s5c Meioc-/- Zygo-like right: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Crest.

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S5c Meioc-/- Zygo-like right

Specimen S6a Meioc+/+ Lepto: postnatal (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Lepto Meioc+/+ Rpa2/Sycp3

Specimen S6a Meioc-/- Lepto: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Lepto Meioc-/- Rpa2/Sycp3

Specimen S6a Meioc+/+ Zygo: postnatal (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Zygo Meioc+/+ Rpa2/Sycp3

Specimen S6a Meioc-/- Zygo-like top: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Zygo-Like Meioc-/- Rpa2/Sycp3 top

Specimen S6a Meioc+/+ Pachy: postnatal (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Pachy Meioc+/+ Rpa2/Sycp3

Specimen S6a Meioc-/- Zygo-like bottom: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Rpa2 (assay MGI:6716297).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6a Zygo-Like Meioc-/- Rpa2/Sycp3 bottom

Specimen S6b Meioc+/+ Lepto: postnatal (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Lepto Meioc+/+ Dmc1/Sycp3

Specimen S6b Meioc-/- Lepto: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Lepto Meioc-/- Dmc1/Sycp3

Specimen S6b Meioc+/+ Zygo: postnatal (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Zygo Meioc+/+ Dmc1/Sycp3

Specimen S6b Meioc-/- Zygo-like top: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Zygo-Like Meioc-/- Dmc1/Sycp3 top

Specimen S6b Meioc+/+ Pachy: postnatal (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Pachy Meioc+/+ Dmc1/Sycp3

Specimen S6b Meioc-/- Zygo-like bottom: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Dmc1 (assay MGI:6716298).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6b Zygo-Like Meioc-/- Dmc1/Sycp3 bottom

Specimen S6c Meioc+/+ Lepto: postnatal (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Lepto Meioc+/+ Rad51/Sycp3

Specimen S6c Meioc-/- Lepto: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Leptotene. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Lepto Meioc-/- Rad51/Sycp3

Specimen S6c Meioc+/+ Zygo: postnatal (more

)
Note: Zygotene. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Zygo Meioc+/+ Rad51/Sycp3

Specimen S6c Meioc-/- Zygo-like top: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Zygo-Like Meioc-/- Rad51/Sycp3 top

Specimen S6c Meioc+/+ Pachy: postnatal (more

)
Note: Pachytene. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Pachy Meioc+/+ Rad51/Sycp3

Specimen S6c Meioc-/- Zygo-like bottom: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Zygotene-like. Double labeled: red - Sycp3; green - Rad51 (assay MGI:6716303).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present		S6c Zygo-Like Meioc-/- Rad51/Sycp3 bottom

S7a Meioc+/+ ©

Specimen S7a Meioc+/+: postnatal day 16 (more

)
Note: Double labeled: red - Sycp3; green - phospho-Histone3.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		S7a Meioc+/+

S7a Meioc-/- ©

Specimen S7a Meioc-/-: postnatal day 16; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - phospho-Histone3.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		S7a Meioc-/-

Specimen S7c Meioc-/- top: postnatal day 17; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - BrdU.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		S7c merge upper left , S7c Sycp3 upper

Specimen S7c Meioc-/- bottom: postnatal day 17; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - BrdU.

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		S7c merge lower left , S7c Sycp3 lower

Specimen S7d -/-M*: postnatal; Meioc^{tm1a(KOMP)Wtsi}/Meioc^{tm1a(KOMP)Wtsi} (more

)
Note: Double labeled: red - Sycp3; green - phospho-Histone3.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S7d merge upper left , S7d Sycp3 upper

Specimen S7d +/+ Mitotic M: postnatal (more

)
Note: Double labeled: red - Sycp3; green - phospho-Histone3.

Structure	Level	Pattern	Image	Note
TS28: testis	Present		S7d merge lower left , S7d Sycp3 lower

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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