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Gene Expression Data
Assay Details
Assay
Reference: J:286303 Buckley DM, et al., Gbx1 and Gbx2 Are Essential for Normal Patterning and Development of Interneurons and Motor Neurons in the Embryonic Spinal Cord. J Dev Biol. 2020 Apr 1;8(2)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6705111
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: clone 39.4D5
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 5A: embryonic day 13.5 (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 5A Expression was detected in motor neurons in the ventral spinal cord. None of the Isl1-positive cells were co-labeled with Casp3.
Specimen 5B: embryonic day 13.5; Gbx1tm1.1Stwt/Gbx1tm1.1Stwt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 5B Expression was detected in motor neurons in the ventral spinal cord. Some of the Isl1-positive cells were co-labeled with Casp3 in the Gbx1 mutant.
Specimen 5C: embryonic day 13.5; Gbx2tm1.1Mrt/Gbx2tm1.1Mrt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 5C Expression was detected in motor neurons in the ventral spinal cord. Some of the Isl1-positive cells were co-labeled with Casp3 in the Gbx2 mutant at E13.5.
Specimen 5D: embryonic day 13.5; Gbx1tm1.1Stwt/Gbx1tm1.1Stwt, Gbx2tm1.1Mrt/Gbx2tm1.1Mrt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS21: spinal cord Present Regionally restricted 5D Expression was detected in motor neurons in the ventral spinal cord. Some of the Isl1-positive cells were co-labeled with Casp3 in the Gbx dKO mutant.
Specimen 5E: embryonic day 14.5 (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5E Expression was detected in motor neurons in the ventral spinal cord. None of the Isl1-positive cells were co-labeled with Casp3.
Specimen 5F: embryonic day 14.5; Gbx1tm1.1Stwt/Gbx1tm1.1Stwt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5F Expression was detected in motor neurons in the ventral spinal cord. Some of the Isl1-positive cells were co-labeled with Casp3 in the Gbx1 mutant.
Specimen 5G: embryonic day 14.5; Gbx2tm1.1Mrt/Gbx2tm1.1Mrt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5G Expression was detected in motor neurons in the ventral spinal cord. None of the Isl1-positive cells were co-labeled with Casp3 in the Gbx2 mutant.
Specimen 5H: embryonic day 14.5; Gbx1tm1.1Stwt/Gbx1tm1.1Stwt, Gbx2tm1.1Mrt/Gbx2tm1.1Mrt (more )
Note: Lumber spinal cord. Double labeled: red - Isl1; green - Casp3 (assay MGI:6705110).
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 5H Expression was detected in motor neurons in the ventral spinal cord. Some of the Isl1-positive cells were co-labeled with Casp3 in the Gbx1 mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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