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Gene Expression Data
Assay Details
Assay
Reference: J:231231 Abby E, et al., Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6715942
Gene symbol: Sycp3
Gene name: synaptonemal complex protein 3
Antibody: Anti-Sycp3 (NB300-232)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Two antibodies were described MGI:5805440 and MGI:6694240; either could have been used in this assay.
Results
Specimen 5a +/+ VASA/SYCP3 18.5-dpc: embryonic day 18.5 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS26: germ cell of testis Absent 5a 18.5dpc Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 18.5-dpc: embryonic day 18.5; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS26: germ cell of testis Absent 5a 18.5dpc Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 6-dpp: postnatal day 6 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 5a 6dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 6-dpp: postnatal day 6; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Absent 5a 6dpp Meioc-/- Vasa/Sycp3
Specimen 5a +/+ VASA/SYCP3 8-dpp: postnatal day 8 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 8dpp Meioc+/+ Vasa/Sycp3 Meiosis initiated at 8 dpp.
Specimen 5a -/- VASA/SYCP3 8-dpp: postnatal day 8; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Weak 5a 8dpp Meioc-/- Vasa/Sycp3 At 8 dpp Sycp3 appeared to be globally weaker in the mutant than in wild type testes.
Specimen 5a +/+ VASA/SYCP3 10-dpp: postnatal day 10 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 10dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 10-dpp: postnatal day 10; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 10dpp Meioc-/- Vasa/Sycp3 A progressive accumulation of Sycp3-positive cells was noted in later stages.
Specimen 5a +/+ VASA/SYCP3 16-dpp: postnatal day 16 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 16dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 16-dpp: postnatal day 16; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 16dpp Meioc-/- Vasa/Sycp3 A progressive accumulation of Sycp3-positive cells was noted in later stages.
Specimen 5a +/+ VASA/SYCP3 20-dpp: postnatal day 20 (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 20dpp Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 20-dpp: postnatal day 20; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a 20dpp Meioc-/- Vasa/Sycp3 A progressive accumulation of Sycp3-positive cells was noted in later stages.
Specimen 5a +/+ VASA/SYCP3 Adult: postnatal adult (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a Adult Meioc+/+ Vasa/Sycp3
Specimen 5a -/- VASA/SYCP3 Adult: postnatal adult; Meioctm1a(KOMP)Wtsi/Meioctm1a(KOMP)Wtsi (more )
Note: Double labeled: brown - Sycp3; magenta - Ddx4 (assay MGI:6695761).
Structure Level Pattern Image Note
TS28: germ cell of testis Present 5a Adult Meioc-/- Vasa/Sycp3 A progressive accumulation of Sycp3-positive cells was noted in later stages.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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