Gene Expression Data
Assay Details
Assay
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Reference:
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J:305110
Xu X, et al., A secreted protein promotes cleavage furrow maturation during cytokinesis. Curr Biol. 2011 Jan 25;21(2):114-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6716054
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Gene symbol:
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Hmcn2
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Gene name:
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hemicentin 2
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Antibody:
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anti-hemicentrin
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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The antibody used in this assay was raised against two family members.
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Results
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Specimen
2A/C:
embryonic day 0.5
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2A/C:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Specimen
2E/G:
embryonic day 1.0
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2E/G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Specimen
2I/K:
embryonic day 1.0
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2I/K:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
|
Note:
|
Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Specimen
2M/O:
embryonic day 1.5
(more )
Note: Three-cell embryo. Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2M/O:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 1.5
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
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Note:
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Three-cell embryo. Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Specimen
2Q P1:
embryonic day 1.0
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2Q P1:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Formaldehyde
|
Note:
|
Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
Punctate |
2Q P1
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Expression is in a punctate open ring around the periphery of the cleavage furrow.
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Specimen
2Q P2:
embryonic day 1.0
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2Q P2:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
Punctate |
2Q P2
|
Expression is in a punctate open ring around the periphery of the cleavage furrow.
|
Specimen
2Q P3:
embryonic day 1.0
(more )
Note: Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
Specimen
2Q P3:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 1.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
Formaldehyde
|
Note:
|
Double labeled: red - Hmcn1 and Hmcn2; green - Myh10 (assay MGI:6716048).
|
Structure |
Level |
Pattern |
Image |
Note |
TS1: 1-cell stage embryo |
Present |
Punctate |
2Q P3
|
Expression is in a punctate open ring around the periphery of the cleavage furrow.
|
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