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Gene Expression Data
Assay Details
Assay
Reference: J:253477 Bailey AS, et al., The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. Elife. 2017 Oct 31;6:e26116
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6724564
Gene symbol: Ccna2
Gene name: cyclin A2
Antibody: anti-Cyclin A2 (EPR17351, ab181591)
Detection system: Alexa Fluor
Results
Specimen 7A: postnatal day 12 (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatogonium Strong Regionally restricted 7A' , 7A Highly expression in spermatogonia undergoing mitotic divisions along the periphery of tubules. Expression fully down-regulated in germ cells by entry into leptotene stage.
Specimen 7B: postnatal day 12; Ythdc2tm1b(EUCOMM)Hmgu/Ythdc2tm1b(EUCOMM)Hmgu (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Strong Regionally restricted 7B' , 7B Expression remained high in germ cells typical of leptotene spermatocytes.
Specimen 7C: postnatal day 12 (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Weak Regionally restricted 7C Expression fully down-regulated in germ cells by entry into leptotene stage.
Specimen 7D: postnatal day 12; Ythdc2tm1b(EUCOMM)Hmgu/Ythdc2tm1b(EUCOMM)Hmgu (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Strong Regionally restricted 7D High expression in preleptotene spermatocytes that moved inward from periphery of tubules.
Specimen 7-S1A: postnatal day 12 (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: germ cell of testis Weak Regionally restricted 7 S1A' , 7 S1A Expression decreased substantially in preleptotene cells.
Specimen 7-S1B: postnatal day 12; Ythdc2tm1b(EUCOMM)Hmgu/Ythdc2tm1b(EUCOMM)Hmgu (more )
Note: Triple labeled: green - Cyclin A2 (Ccna2); red - Sycp3 (assay MGI:6724544); blue - DAPI.
Structure Level Pattern Image Note
TS28: spermatocyte Strong Regionally restricted 7 S1B , 7 S1B' High expression in preleptotene spermatocytes that moved inward from periphery of tubules.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory