Gene Expression Data
Assay Details
Assay
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Reference:
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J:130321
Sato H, et al., Visualizing expression patterns of Shh and Foxf1 genes in the foregut and lung buds by optical projection tomography. Pediatr Surg Int. 2008 Jan;24(1):3-11
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6728870
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh probe198
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 9.0
(more )
Specimen
1A:
(close )
Genetic Background:
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CBA/Ca
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Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS14: embryo |
Present |
|
1A
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Authors state the precise domain of ventral localization could not be ascertained from whole mounts.
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Specimen
1B:
embryonic day 9.0
(more )
Note: Imaged by optical projection tomography (OPT).
Specimen
1B:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Imaged by optical projection tomography (OPT).
|
Specimen
1C:
embryonic day 9.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1C:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut |
Present |
Regionally restricted |
1C
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Expression localized to ventral aspect of foregut invagination at level of branchial arches.
|
Specimen
1D:
embryonic day 9.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1D:
(close )
Genetic Background:
|
CBA/Ca
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Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut |
Present |
Regionally restricted |
1D
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Expression ventrally restricted in foregut.
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Specimen
1E:
embryonic day 9.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1E:
(close )
Genetic Background:
|
CBA/Ca
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Age:
|
embryonic day 9.0
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Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: foregut |
Present |
Regionally restricted |
1E
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Expression ventrally restricted in foregut.
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Specimen
1F:
embryonic day 10.0
(more )
Specimen
1F:
(close )
Genetic Background:
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CBA/Ca
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Age:
|
embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Specimen
1G:
embryonic day 10.0
(more )
Note: Imaged by optical projection tomography (OPT).
Specimen
1G:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 10.0
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Sex:
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Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Agarose
|
Note:
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Imaged by optical projection tomography (OPT).
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: laryngo-tracheal groove |
Present |
Regionally restricted |
1G
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Expression restricted to ventral endoderm of laryngotracheal groove, including site of division of repiratory groove from foregut.
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Specimen
1H:
embryonic day 10.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1H:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: lung |
Present |
|
1H
|
|
Specimen
1I:
embryonic day 10.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1I:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: foregut |
Present |
Regionally restricted |
1I
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Ventral expression in anterior foregut.
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Specimen
1J:
embryonic day 10.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1J:
(close )
Genetic Background:
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CBA/Ca
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Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: lung |
Present |
|
1J
|
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Specimen
1K:
embryonic day 11.0
(more )
Specimen
1K:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
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Sex:
|
Not Specified
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Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
1L:
embryonic day 11.0
(more )
Note: Imaged by optical projection tomography (OPT).
Specimen
1L:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Imaged by optical projection tomography (OPT).
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: forelimb bud |
Present |
Regionally restricted |
1L
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Expression in polarizing regions of forelimb and hindlimb buds.
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TS18: hindlimb bud |
Present |
Regionally restricted |
1L
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Expression in polarizing regions of forelimb and hindlimb buds.
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TS18: gut |
Present |
|
1L
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Expression in gut clearly visible with OPT scanning.
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Specimen
1M:
embryonic day 11.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1M:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: laryngo-tracheal groove |
Present |
Regionally restricted |
1M
|
Expression at division of trachea from foregut.
|
Specimen
1N:
embryonic day 11.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1N:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Specimen
1O:
embryonic day 11.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1O:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: respiratory tract |
Present |
Regionally restricted |
1O
|
Expression extending into dorsal terrisory in more caudal sections.
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Specimen
1P:
embryonic day 11.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1P:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: respiratory tract |
Present |
Regionally restricted |
1P
|
Expression extending into dorsal terrisory in more caudal sections.
|
Specimen
1Q:
embryonic day 11.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
1Q:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: lung |
Present |
|
1Q
|
|
Specimen
2A:
embryonic day 12.0
(more )
Specimen
2A:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
2B:
embryonic day 12.0
(more )
Note: Imaged by optical projection tomography (OPT).
Specimen
2B:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Imaged by optical projection tomography (OPT).
|
Specimen
2C:
embryonic day 12.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
2C:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Specimen
2D:
embryonic day 12.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
2D:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Specimen
2E:
embryonic day 12.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
2E:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
Specimen
2F:
embryonic day 12.0
(more )
Note: Image is a virtual section generated from OPT.
Specimen
2F:
(close )
Genetic Background:
|
CBA/Ca
|
Age:
|
embryonic day 12.0
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Agarose
|
Note:
|
Image is a virtual section generated from OPT.
|
|