Pecam1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:287184 Huang K, et al., Annexin A3 is necessary for parallel artery-vein alignment in the mouse retina. Dev Dyn. 2020 May;249(5):666-678
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6729941
Gene symbol:	Pecam1
Gene name:	platelet/endothelial cell adhesion molecule 1

Antibody:

MEC 13.3

Results

2A ©

Specimen 2A: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu} (more

)
Note: Double labeled: red - Pecam1; red - Emcn (assay MGI:6729942).

Structure	Level	Pattern	Image	Note
TS15: intersomitic blood vessel	Present		2A
TS15: vascular system	Present		2A

2A inset ©

Specimen 2A inset: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu} (more

)
Note: Double labeled: red - Pecam1; red - Emcn (assay MGI:6729942).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2A inset
TS15: endocardial lining	Present		2A inset

2B ©

Specimen 2B: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu}, Tg(Tek-cre)1Ywa/0 (more

)
Note: Conditional mutant. Double labeled: red - Pecam1; red - Emcn (assay MGI:6729942).

Structure	Level	Pattern	Image	Note
TS15: vascular system	Present		2B	No gross morphologial defects in vasculature compared with f/f mice.
TS15: intersomitic blood vessel	Present		2B	Expression in intersomitic vessels similar to f/f embryos.

2B inset ©

Specimen 2B inset: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu}, Tg(Tek-cre)1Ywa/0 (more

)
Note: Conditional mutant. Double labeled: red - Pecam1; red - Emcn (assay MGI:6729942).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2B inset	Expression in dorsal aorta and endocardium similar to f/f embryos.
TS15: endocardial lining	Present		2B inset	Expression in dorsal aorta and endocardium similar to f/f embryos.

2C ©

Specimen 2C: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu} (more

)
Note: Triple labeled: red - Pecam1; red - Emcn (assay MGI:6729942); blue - DAPI.

Structure	Level	Image
TS15: dorsal aorta	Present	2C
TS15: vascular system	Present	2C
TS15: gut	Present	2C
TS15: somite	Present	2C

2D ©

Specimen 2D: embryonic day 9.5; Anxa3^{tm1c(EUCOMM)Hmgu}/Anxa3^{tm1c(EUCOMM)Hmgu}, Tg(Tek-cre)1Ywa/0 (more

)
Note: Conditional mutant. Triple labeled: red - Pecam1; red - Emcn (assay MGI:6729942); blue - DAPI.

Structure	Level	Image	Note
TS15: dorsal aorta	Present	2D	No gross defects in dorsal aorta or surrounding blood vessels.
TS15: vascular system	Present	2D	No gross defects in dorsal aorta or surrounding blood vessels.
TS15: gut	Present	2D
TS15: somite	Present	2D

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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