Gene Expression Data
Assay Details
Assay
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Reference:
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J:115783
Kiernan AE, et al., The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet. 2006 Jan;2(1):e4
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6763035
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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anti-Sox2 (AB5603)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
9B:
embryonic day 12.5
(more )
Note: basal region
Specimen
9B:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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basal region
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cochlear duct epithelium |
Present |
Regionally restricted |
9B
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Expression did overlap with the Jag1 in the prosensory domains in the basal regions.
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Specimen
9C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Jag1tm1Grid/Jag1tm2Grid Foxg1tm1(cre)Skm/Foxg1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. basal region
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cochlear duct epithelium |
Weak |
Regionally restricted |
9C
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Expression was absent from the basal regions and significantly downregulated in the apical regions.
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Specimen
9E:
embryonic day 12.5
(more )
Note: apical region
Specimen
9E:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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apical region
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cochlear duct epithelium |
Present |
Regionally restricted |
9E
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In the apical regions, where the sensory precursors have ceased dividing, expression of Jag1 and Sox2 did not overlap.
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Specimen
9F:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Jag1tm1Grid/Jag1tm2Grid Foxg1tm1(cre)Skm/Foxg1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. apical region
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Specimen
10B:
embryonic day 12.5
(more )
Specimen
10B:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
|
Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: semicircular canal |
Present |
Regionally restricted |
10B
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Jag1 and Sox2 exhibited largely overlapping expression in posterior cristae and saccular macula. In the posterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.
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Specimen
10C:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Jag1tm1Grid/Jag1tm2Grid Foxg1tm1(cre)Skm/Foxg1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: semicircular canal |
Present |
Regionally restricted |
10C
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Displayed fairly normal expression in saccular macula. The posterior cristae was missing in the mutant.
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Specimen
10E:
embryonic day 12.5
(more )
Specimen
10E:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: utricle epithelium |
Present |
Regionally restricted |
10E
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Jag1 and Sox2 exhibited largely overlapping expression domains.
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Specimen
10F:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Jag1tm1Grid/Jag1tm2Grid Foxg1tm1(cre)Skm/Foxg1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: utricle epithelium |
Trace |
Regionally restricted |
10F
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Very weak expression and the expression domain was much smaller than in controls.
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Specimen
10H:
embryonic day 12.5
(more )
Specimen
10H:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: semicircular canal |
Present |
Regionally restricted |
10H
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Jag1 and Sox2 exhibited largely overlapping expression in anterior and lateral cristae. In the anterior cristae, expression had a negative patch in the middle, whereas Sox2 expression did not.
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Specimen
10I:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * 129S1/Sv * C57BL/6
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Mutant Allele(s):
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Jag1tm1Grid/Jag1tm2Grid Foxg1tm1(cre)Skm/Foxg1+
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: semicircular canal |
Trace |
Regionally restricted |
10I
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The entire ampullae appeared to be missing or severely disrupted in the mutant.
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