Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:286656
Shylo NA, et al., Loss of ciliary transition zone protein TMEM107 leads to heterotaxy in mice. Dev Biol. 2020 Apr 15;460(2):187-199
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6763181
|
Gene symbol:
|
Nodal
|
Gene name:
|
nodal growth differentiation factor
|
Probe:
|
Nodal cDNA6
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
2G:
embryonic day 8.0
(more )
Note: 2-3 somites
Specimen
2G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-3 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Regionally restricted |
2G
|
Equal bilateral expression.
|
Specimen
2I:
(close )
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
|
Mutant Allele(s):
|
Tmem107schlei/Tmem107tm1Lex
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-3 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Regionally restricted |
2I
|
Randomized expression levels around the mutant nodes.
|
Specimen
2H:
embryonic day 8.0
(more )
Note: 4-5 somites
Specimen
2H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4-5 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Regionally restricted |
2H
|
Reduced expression on the right side.
|
Specimen
2J:
(close )
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
|
Mutant Allele(s):
|
Tmem107schlei/Tmem107tm1Lex
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
4-5 somites
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Regionally restricted |
2J
|
Maintained bilateral expression for longer, and ultimately with a randomized pattern.
|
Specimen
4A:
embryonic day 8.0
(more )
Note: 0-5 somites
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
0-5 somites
|
Specimen
4B:
(close )
Genetic Background:
|
involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
|
Mutant Allele(s):
|
Tmem107schlei/Tmem107tm1Lex
|
Age:
|
embryonic day 8.0
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
0-5 somites
|
|