Gene Expression Data
Assay Details
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Assay
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Reference:
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J:286656
Shylo NA, et al., Loss of ciliary transition zone protein TMEM107 leads to heterotaxy in mice. Dev Biol. 2020 Apr 15;460(2):187-199
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6763181
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Gene symbol:
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Nodal
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Gene name:
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nodal growth differentiation factor
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Probe:
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Nodal cDNA6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2G:
embryonic day 8.0
(more  )
Note: 2-3 somites
Specimen
2G:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-3 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
2G
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Equal bilateral expression.
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tmem107schlei/Tmem107tm1Lex
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-3 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
2I
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Randomized expression levels around the mutant nodes.
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Specimen
2H:
embryonic day 8.0
(more )
Note: 4-5 somites
Specimen
2H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
2H
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Reduced expression on the right side.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tmem107schlei/Tmem107tm1Lex
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-5 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: node |
Present |
Regionally restricted |
2J
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Maintained bilateral expression for longer, and ultimately with a randomized pattern.
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Specimen
4A:
embryonic day 8.0
(more )
Note: 0-5 somites
Specimen
4A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-5 somites
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S5/SvEvBrd * C57BL/6J * C57BL/6N
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Mutant Allele(s):
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Tmem107schlei/Tmem107tm1Lex
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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0-5 somites
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Analysis Tools