Hmgcr RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:306194 Ghimire S, et al., PEG3 controls lipogenesis through ACLY. PLoS One. 2021;16(5):e0252354
Assay type:	RT-PCR
MGI Accession ID:	MGI:6780739
Gene symbol:	Hmgcr
Gene name:	3-hydroxy-3-methylglutaryl-Coenzyme A reductase

Probe:	Hmgcr-pG, Hmgcr-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR was used to obtain these results; Gapdh was used to normalize expression levels.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
WT-F1	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
CoKO-F1	P adult	TS28: mammary gland	Present (a)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Female	gene dosage of Peg3 is almost zero
WT-F2	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Female
CoKO-F2	P adult	TS28: mammary gland	Present (b)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Female	gene dosage of Peg3 is almost zero
WT-M1	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Male
CoKO-M1	P adult	TS28: mammary gland	Present (c)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Male	gene dosage of Peg3 is almost zero
WT-M2	P adult	TS28: mammary gland	Present	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N		Male
CoKO-M2	P adult	TS28: mammary gland	Present (d)	Not Specified; total RNA	involves: C57BL/6J * C57BL/6N	Peg3^{tm1a(EUCOMM)Hmgu}/Peg3⁺	Male	gene dosage of Peg3 is almost zero

Notes:
(a) Expression levels are increased 2.1 fold in the mutant.
(b) Expression levels are increased 1.4 fold in the mutant.
(c) Expression levels are reduced 60% in the mutant.
(d) Expression levels are increased 1.7 fold in the mutant.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 12/10/2024 MGI 6.24