Sftpc Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:243650 Herriges MJ, et al., The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. Genes Dev. 2017 May 01;31(9):889-903
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6863924
Gene symbol:	Sftpc
Gene name:	surfactant associated protein C

Antibody:	anti-Sftpc
Detection system:	Secondary antibody

Results

1F top Lineage Marker ©

1F top merge ©

Specimen 1F - Sftpc: postnatal adult; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Sftpc; red - Sfta3-ps reporter (assay MGI:6863897).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	1F top Lineage Marker , 1F top merge	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells.

1H top Lineage Marker ©

1H top merge ©

Specimen 1H - Sftpc: postnatal adult (more

)
Note: Double labeled: green - Sftpc; red - Nkx2-1 (assay MGI:6863898).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	1H top Lineage Marker , 1H top merge	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells.

3B lower Wild-type ©

Specimen 3B - Wild-type: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B lower Wild-type	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells.

3B lower NANCI^RFP/+ ©

Specimen 3B - NANCI^{creERT2-RFP/+}: embryonic day 18.5; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B lower NANCI^RFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are comparable to those seen in wild type.

3B lower Nkx2.1^GFP/+ ©

Specimen 3B - Nkx2.1^GFP/+: embryonic day 18.5; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B lower Nkx2.1^GFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are significantly reduced compared to those seen in wild type.

3B lower NANCI^RFP/+; Nkx2.1^GFP/+ ©

Specimen 3B - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: embryonic day 18.5; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS26: lung epithelium	Present		3B lower NANCI^RFP/+; Nkx2.1^GFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are significantly reduced compared to those seen in wild type.

3E lower Wild-type ©

Specimen 3E - Wild-type: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E lower Wild-type	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells.

3E lower NANCI^RFP/+ ©

Specimen 3E - NANCI^{creERT2-RFP/+}: postnatal day 4; Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E lower NANCI^RFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are reduced compared to those seen in wild type.

3E lower Nkx2.1^GFP/+ ©

Specimen 3E - Nkx2.1^GFP/+: postnatal day 4; Nkx2-1^tm1Ikono/Nkx2-1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E lower Nkx2.1^GFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are reduced compared to those seen in wild type.

Specimen 3E - NANCI^{creERT2-RFP/+}; Nkx2.1^GFP/+: postnatal day 4; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present		3E lower NANCI^RFP/+; Nkx2.1^GFP/+	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; their numbers are significantly reduced compared to those seen in wild type.

5E ©

Specimen 5E: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Sftpc; red - Sox2 (assay MGI:6863946).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	5E	Expression is seen in a small subset of the bronchiolized cells in the distal lung.

5F ©

Specimen 5F: postnatal week 10; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: red - Sftpc; green - Scgb1a1 (assay MGI:6863929).

Structure	Level	Pattern	Image	Note
TS28: lung epithelium	Present	Single cells	5F	Expression is seen in a small subset of the bronchiolized cells in the distal lung.

S8J ©

Specimen S8J: postnatal month 6; Nkx2-1^tm1Ikono/Nkx2-1⁺, Sfta3^{tm1.1(cre/ERT2)Eem}/Sfta3⁺ (more

)
Note: Double labeled: green - Sftpc; red - Mki67 (assay MGI:6863973).

Structure	Level	Pattern	Image	Note
TS28: lung	Present		S8J	Expression is seen in pulmonary epithelium alveolar type 2 (AT2) cells; they are not showing an increase in proliferation in the mutant. This is quantified in Fig. S8K.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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