Gene Expression Data
Assay Details
Assay
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Reference:
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J:237701
Saare M, et al., Expression Pattern and Localization Dynamics of Guanine Nucleotide Exchange Factor RIC8 during Mouse Oogenesis. PLoS One. 2015;10(6):e0129131
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6864134
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Gene symbol:
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Ric8a
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Gene name:
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RIC8 guanine nucleotide exchange factor A
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Antibody:
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anti-Ric8
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Detection system:
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Alexa Fluor
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Results
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Specimen
1A:
postnatal adult
(more )
Note: primordial follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Note:
|
primordial follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Specimen
1B:
postnatal adult
(more )
Note: primary follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
primary follicle stage. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Specimen
1C:
postnatal adult
(more )
Note: preantral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
preantral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte of secondary follicle |
Present |
Regionally restricted |
1C
|
At preantral secondary follicle stage, cytoplasm localization of expression in oocyte.
|
Specimen
1D:
postnatal adult
(more )
Note: antral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1D:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
antral secondary follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte of secondary follicle |
Present |
Regionally restricted |
1D
|
At antral secondary follicle stage, cytoplasm localization of expression in oocyte. Expression also targeted to germical vesicle.
|
Specimen
1E:
postnatal adult
(more )
Note: Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte of mature follicle |
Present |
Regionally restricted |
1E
|
At preovulatory stage, expression localized in germinal vesicle. Expression accumulates from cytoplasm to cell cortex.
|
TS28: cumulus of mature follicle |
Present |
Regionally restricted |
1E
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Expression also present at detectable level in cytoplasm of surrounding cumulus cells
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Specimen
1F:
postnatal adult
(more )
Note: Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1F:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Graafian follicle. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte of mature follicle |
Present |
Regionally restricted |
1F
|
Expression accumulates from cytoplasm to cell cortex.
|
TS28: cumulus of mature follicle |
Present |
Regionally restricted |
1F
|
Expression also present at detectable level in cytoplasm of surrounding cumulus cells and mural granulosa cells.
|
TS28: mural of mature follicle |
Present |
Regionally restricted |
1F
|
Expression also present at detectable level in cytoplasm of surrounding cumulus cells and mural granulosa cells.
|
Specimen
1G:
postnatal adult
(more )
Note: Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1G:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oviduct |
Present |
Widespread |
1G
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Expression in all regions of Fallopian tube.
|
Specimen
1H:
postnatal adult
(more )
Note: ampulla region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1H:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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ampulla region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Specimen
1I:
postnatal adult
(more )
Note: isthmus region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1I:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
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isthmus region. Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oviduct isthmus |
Strong |
Regionally restricted |
1I
|
High level of expression in epithelium of isthmus.
|
Specimen
1i:
postnatal adult
(more )
Note: Double labeled: red - RIC8 (Ric8a); blue - DAPI.
Specimen
1i:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - RIC8 (Ric8a); blue - DAPI.
|
Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: endometrium |
Weak |
|
|
Expression in endometrium of uterus at markedly lower level.
|
Specimen
2A:
postnatal adult
(more )
Note: partial rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
partial rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
2A
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When chromatic starts to condense as a partial rim around the nucleolus, expression localized to germinal vesicle.
|
Specimen
2a:
postnatal adult
(more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2a:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Diffuse |
2a
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Diffuse expression across germinal vesicle.
|
Specimen
2B:
postnatal adult
(more )
Note: full rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
full rim stage. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Spotted |
2B
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Expression accumulated in spots distributed around condensed chromatin in germinal vesicle.
|
Specimen
2b:
postnatal adult
(more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2b:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
2b
|
Some foci of expression attached to nucleolus.
|
Specimen
2C:
postnatal adult
(more )
Note: disappearance of nucleolus. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
disappearance of nucleolus. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
2C
|
When nuclear lamina and nucleolus start to disappear, expression remains in vicinity of chromosomes.
|
Specimen
2c:
postnatal adult
(more )
Note: Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2c:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Spotted |
2c
|
Spots of expression in close vicinity of chromosomes.
|
Specimen
2E:
postnatal adult
(more )
Note: metaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
metaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
2E
|
With organization of chromosomes to the metaphase plate, expression distributed along metaphase spindle, colocalized with tubulin.
|
Specimen
2G:
postnatal adult
(more )
Note: anaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2G:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
anaphase of meiosis I. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: oocyte |
Present |
Regionally restricted |
2G
|
During chromosome separation, expression remained in vicinity of chromosomes, locallized with meiotic spindle, colocalized with tubulin.
|
Specimen
2I:
postnatal adult
(more )
Note: metaphase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2I:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
metaphase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: secondary oocyte |
Present |
Regionally restricted |
2I
|
With organization of chromosomes to the metaphase plate, distributed along metaphase spindle, colocalized with tubulin.
|
Specimen
2K:
postnatal adult
(more )
Note: telophase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
Specimen
2K:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
telophase of meiosis II. Triple labeled: red - RIC8 (Ric8a); green - Ph (F-actin stained with phalloidin); blue - DAPI.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: secondary oocyte |
Present |
Regionally restricted |
2K
|
During final steps of meiosis II, expression located in meiotic spindle, colocalized with tubulin.
|
|