Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:306594
De Maria A, et al., Expression of potassium-dependent sodium-calcium exchanger in the murine lens. Exp Eye Res. 2018 Feb;167:18-24
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6865730
|
Gene symbol:
|
Slc24a4
|
Gene name:
|
solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
|
Probe:
|
Mm-Slc24a4 (313991)
|
Probe preparation:
|
Antisense labelled with alkaline phosphatase
|
Visualized with:
|
Chromogenic
|
|
Results
|
Specimen
E12 not shown:
embryonic day 12.0
(more )
Specimen
E12 not shown:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
2A:
embryonic day 16.5
(more )
Specimen
2A:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: lens fiber |
nucleated secondary lens fiber |
Strong |
Regionally restricted |
2A
|
Abundant expression in nucleated secondary lens fiber cells.
|
TS24: lens fiber |
non-nucleated secondary lens fiber |
Weak |
Regionally restricted |
2A
|
Low expression in central fiber cells undergoing undergoing nuclear degradation.
|
TS24: lens epithelium |
lens epithelial cell |
Trace |
|
2A
|
Almost undetectable expression in epithelial cells.
|
Specimen
2B:
postnatal day 30
(more )
Specimen
2B:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal day 30
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
2B
|
Abundant expression in lens fiber cells, restricted to fibers in outer lens cortex. No expression in inner cortical region bordering the organelle-free zone (OFZ)
|
TS28: lens |
|
Present |
Regionally restricted |
2B
|
No expression in cells of organelle-free zone (OFZ) itself.
|
TS28: lens epithelium |
lens epithelial cell |
Trace |
|
2B
|
Almost undertectable expression in epithelial cells.
|
|