Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:306594
De Maria A, et al., Expression of potassium-dependent sodium-calcium exchanger in the murine lens. Exp Eye Res. 2018 Feb;167:18-24
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6865731
|
Gene symbol:
|
Slc24a4
|
Gene name:
|
solute carrier family 24 (sodium/potassium/calcium exchanger), member 4
|
Antibody:
|
Nckx4 antibody
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Results
|
Specimen
3A/C:
postnatal adult
(more )
Specimen
3A/C:
(close )
Genetic Background:
|
C57BL/6J
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: lens |
non-nucleated secondary lens fiber |
Present |
Regionally restricted |
3A
,
3C
|
Expression in both nucleated and non-nucleated regions of lens cortex.
|
TS28: lens |
nucleated secondary lens fiber |
Present |
Regionally restricted |
3A
,
3C
|
Expression in both nucleated and non-nucleated regions of lens cortex.
|
TS28: lens fiber |
lens fiber cell |
Present |
Regionally restricted |
3A
,
3C
|
Expression also in fiber cells within organelle-free zone (OFZ).
|
TS28: lens epithelium |
lens epithelial cell |
Absent |
|
3C
,
3A
|
Near absent expression in lens epithelium.
|
Specimen
3D/F:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac * FVB/N
|
Mutant Allele(s):
|
Slc24a4tm1.2Hazh/Slc24a4tm1.2Hazh Tg(Cryaa-cre)10Mlr/0
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Conditional mutant.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lens |
Absent |
|
3F
,
3D
|
Expression reduced to low, background levels.
|
|