Antibody:	anti-Runx2
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Uniform	6A Ankrd11ctrl Runx2

Structure	Level	Pattern	Image	Note
TS22: maxilla	Present	Graded	6A Ankrd11ncko Runx2	Expression is strong in the center and becomes diffuse toward the periphery. Quantification of the expression domains indicated that expression was more contained, but the differences in the area occupied did not reach statistical significance.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: maxilla	osteoblast	Present		6C Ankrd11ctrl Runx2 left , 6C Ankrd11ctrl Runx2 right	Expression is in osteoblasts lining the newly formed trabeculae.

Structure	Level	Pattern	Image	Note
TS27: maxilla	Present	Regionally restricted	6C Ankrd11ncko Runx2 left , 6C Ankrd11ncko Runx2 right	Expression is patchy and more restricted to the small sparse trabeculae. There was a 85% reduction of the expression domain as well as 76% reduction in the number of positive cells compared to controls.