Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218166 Zhulyn O, et al., Ptch2 shares overlapping functions with Ptch1 in Smo regulation and limb development. Dev Biol. 2015 Jan 15;397(2):191-202
Assay type:	RNA in situ
MGI Accession ID:	MGI:7256489
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe207
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

S3F ©

Specimen S3F: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present		S3F

S3G ©

Specimen S3G: embryonic day 10.5; Ptch2^tm1Hui/Ptch2^tm1Hui (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present		S3G	Normal anterior-posterior polarity

4D ©

Specimen 4D: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present		4D

4E ©

Specimen 4E: embryonic day 11.5; Ptch1^tm1Bjw/Ptch1^tm1.1Bjw, Tg(Msx2-cre)5Rem/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: limb bud	Present		4E

4F ©

Specimen 4F: embryonic day 11.5; Ptch1^tm1Bjw/Ptch1^tm1Mps, Ptch2^tm1Bjw/Ptch2^tm1Bjw, Tg(Msx2-cre)5Rem/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS19: limb bud	Weak		4F	Decreased expression upon loss of Ptch1 and Ptch2.

7G ©

Specimen 7G: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present		7G

7I ©

Specimen 7I: embryonic day 10.5; Ptch1^tm1Bjw/Ptch1^tm1.1Bjw, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	7I	Abnormal expression at periphery of limb.

7K ©

Specimen 7K: embryonic day 10.5; Ptch1^tm1Bjw/Ptch1^tm1.1Bjw, Ptch2^tm1Hui/Ptch2^tm1Hui, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: limb bud	Present	Regionally restricted	7K	Abnormal expression at periphery of limb.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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