Gene Expression Data
Assay Details
Assay
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Reference:
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J:257920
Tahara N, et al., Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules. Dev Biol. 2018 Feb 1;434(1):74-83
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7256712
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Probe:
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isl1 probe40
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1C:
embryonic day 9.5
(more )
Specimen
1C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3E:
embryonic day 9.5
(more )
Note: 25 somite stage
Specimen
3E:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 9.5
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
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Note:
|
25 somite stage
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Specimen
3F:
embryonic day 9.5
(more )
Note: 26 somite stage
Specimen
3F:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
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26 somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: hindlimb ridge |
Present |
Regionally restricted |
3F
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Expression localized to posterior of hindlimb bud during hindlimb budding.
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Specimen
3G:
embryonic day 9.5
(more )
Note: 28 somite stage
Specimen
3G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
28 somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: hindlimb ridge |
Present |
Regionally restricted |
3G
|
Expression localized to posterior of hindlimb bud during hindlimb budding.
|
Specimen
3H:
embryonic day 10.0
(more )
Note: 30 somite stage
Specimen
3H:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 10.0
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Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
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Fixation:
|
4% Paraformaldehyde
|
Note:
|
30 somite stage
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: hindlimb bud |
Trace |
Regionally restricted |
3H
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Very faint expression in hindlimb bud.
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