Gene Expression Data
Assay Details
Assay
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Reference:
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J:110714
Barrionuevo F, et al., Sox9 is required for notochord maintenance in mice. Dev Biol. 2006 Jul 1;295(1):128-40
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7258382
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Gene symbol:
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Pax1
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Gene name:
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paired box 1
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Probe:
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Pax1 probe39
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Assay notes:
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Two probes were described, but the one used in this assay was not detailed. Pax1 cDNA8 MGI:7258349 may have been used in this assay.
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Results
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Specimen
4V:
embryonic day 10.5
(more )
Specimen
4V:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4V
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Expression in ventro-medial sclerotome.
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Specimen
4W:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Sox9tm1Gsr/Sox9tm1Gsr Krt19tm1(cre)Mmt/Krt19+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS17: sclerotome |
Present |
Regionally restricted |
4W
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Expression unchanged in mutant compared with wild type.
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Specimen
5Q:
embryonic day 11.5
(more )
Specimen
5Q:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: sclerotome |
Strong |
Regionally restricted |
5Q
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Highly expressed in presumptive intervertebral region.
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Specimen
5R:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Sox9tm1Gsr/Sox9tm1Gsr Krt19tm1(cre)Mmt/Krt19+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: sclerotome |
Present |
Regionally restricted |
5R
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Expression maintained in sclerotome, but in an unsegmental manner.
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Specimen
6J:
embryonic day 13.5
(more )
Specimen
6J:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: sclerotome |
Present |
Regionally restricted |
6J
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Expression in narrow zones of intervertebral regions.
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Specimen
6N:
(close )
Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Sox9tm1Gsr/Sox9tm1Gsr Krt19tm1(cre)Mmt/Krt19+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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Structure |
Level |
Pattern |
Image |
Note |
TS21: sclerotome |
Present |
Regionally restricted |
6N
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Expression in mesenchyme surrounding cartilage cores.
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