Antibody:	anti-Sox9
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		1E
TS19: sclerotome	Present	Regionally restricted	1E	Expression in prospective vertebral regions.

Structure	Level	Pattern	Image	Note
TS21: notochord	Present		1G
TS21: sclerotome	Present	Regionally restricted	1G	Expression in prospective vertebral regions during chondrogenesis.

Structure	Level	Pattern	Image	Note
TS22: vertebral cartilage condensation	Present		1I	Expression in all vertebral chondrocytes at E14.5.

Structure	Level	Pattern	Image	Note
TS23: vertebral cartilage condensation	Present	Regionally restricted	1J	No expression in hypertrophic chondrocytes of vertebral core at E15.5.

Structure	Level	Pattern	Image	Note
TS22: annulus fibrosus	Present	Regionally restricted	1L	Expression in cartilaginous inner annulus.
TS22: nucleus pulposus	Present		1L	Expression in notochord-derived nucleus pulposus.

Structure	Level	Pattern	Image	Note
TS23: annulus fibrosus	Present	Regionally restricted	1M	Expression in cartilaginous inner annulus.
TS23: nucleus pulposus	Weak		1M	Number of expressing cells in nucleus pulposus is strongly reduced.

Structure	Level	Pattern	Image	Note
TS13: notochord	Present		2C

Structure	Level	Pattern	Image	Note
TS13: notochord	Present	Scattered	2D	Expression largely absent except for some expressing notochord cells scattered along its anterior-posterior extenstion.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		5E
TS19: sclerotome	Present	Regionally restricted	5E	Expression in less condensed mesenchymal regions fated to become vertebral bodies.

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		5F	Expression only in remaining notochord cells.
TS19: sclerotome	Present	Regionally restricted	5F	No expression in slerotomal cells along entire anterior-posterior extension of peri-notochordal area, except for most distal regions.