Cdh5 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316418 Azzoni E, et al., The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition. Cell Rep. 2021 Dec 14;37(11):110103
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7276242
Gene symbol:	Cdh5
Gene name:	cadherin 5

Antibody:	anti-VECAD (BV13)
Detection system:	Secondary antibody

Results

1A VE-Cad/DLL4 +/+ (left) ©

Specimen 1A +/+ VE-Cad: embryonic day 8.25 (more

)
Note: 4-6 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present		1A VE-Cad/DLL4 +/+ (left)
TS12: vitelline artery	Present		1A VE-Cad/DLL4 +/+ (left)

1A VE-Cad/DLL4 -/- (left) ©

Specimen 1A -/- VE-Cad: embryonic day 8.25; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 4-6 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present		1A VE-Cad/DLL4 -/- (left)
TS12: vitelline artery	Present		1A VE-Cad/DLL4 -/- (left)

1B +/+ (left) ©

Specimen 1B +/+ VE-Cad: embryonic day 9.5 (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); green - Runx1 (assay MGI:7276241).

Structure	Level	Image
TS15: dorsal aorta	Present	1B +/+ (left)
TS15: umbilical artery	Present	1B +/+ (left)
TS15: vitelline artery	Present	1B +/+ (left)

1B -/- (left) ©

Specimen 1B -/- VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); green - Runx1 (assay MGI:7276241).

Structure	Level	Image
TS15: dorsal aorta	Present	1B -/- (left)
TS15: umbilical artery	Present	1B -/- (left)
TS15: vitelline artery	Present	1B -/- (left)

1C +/+ (left) ©

Specimen 1C +/+ VE-Cad: embryonic day 9.5 (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Image
TS15: dorsal aorta	Present	1C +/+ (left)
TS15: umbilical artery	Present	1C +/+ (left)
TS15: vitelline artery	Present	1C +/+ (left)

1C -/- (left) ©

Specimen 1C -/- VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		1C -/- (left)
TS15: vitelline artery	Present		1C -/- (left)

1C +/+ (middle right) ©

Specimen 1C +/+ slice VE-Cad: embryonic day 9.5 (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		1C +/+ (middle right)

1C -/- (middle right) ©

Specimen 1C -/- slice VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 22-26 somite pairs. Double labeled: white - VE-Cad (Cdh5); red - Dll4 (assay MGI:7276243).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		1C -/- (middle right)

2A VE-Cad/CD43/Runx1 +/+ (left) ©

Specimen 2A +/+ (left) VE-Cad: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A VE-Cad/CD43/Runx1 +/+ (left)	Expression in hemogenic endothelium.

2A VE-Cad/CD43/runx1 -/- (left) ©

Specimen 2A -/- (left) VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta endothelium	Present	Regionally restricted	2A VE-Cad/CD43/runx1 -/- (left)	Expression in hemogenic endothelium.

2A VE-Cad/CD43/Runx1 +/+ (right) ©

Specimen 2A +/+ (right) VE-Cad: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Cell Type	Level	Pattern	Image	Note
TS15: vitelline artery	hematopoietic cell	Present		2A VE-Cad/CD43/Runx1 +/+ (right)

Specimen 2A -/- (right) VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: vitelline artery	Present	Regionally restricted	2A VE-Cad/CD43/Runx1 -/- (right)	Expression in hemogenic endothelium.

Specimen 2F +/+ VE-Cad: embryonic day 9.5 (more

)
Note: 23-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - Jag1 (assay MGI:7276246); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2F VE-Cad/Jag1/Runx1 +/+

Specimen 2F -/- VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 23-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - Jag1 (assay MGI:7276246); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Present		2F VE-Cad/Jag1/Runx1 -/-

Specimen S1D +/+ VE-Cad: embryonic day 9.5 (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D VE-Cad/CD43/Runx1 +/+

Specimen S1D -/- VE-Cad: embryonic day 9.5; Slc8a1^tm1Sjc/Slc8a1^tm1Sjc (more

)
Note: 21-26 somite pairs. Triple labeled: white - VE-Cad (Cdh5); red - CD43 (Spn assay MGI:7276244); green - Runx1 (assay MGI:7276241).

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Present		S1D VE-Cad/CD43/Runx1 -/-

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