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Gene Expression Data
Assay Details
Assay
Reference: J:321982 De A, et al., The beta8 integrin cytoplasmic domain activates extracellular matrix adhesion to promote brain neurovascular development. Development. 2022 Mar 15;149(6):dev200472
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7281830
Gene symbol: Smad3
Gene name: SMAD family member 3
Antibody: anti-Smad3 (phospho S423 + S425) (abcam52903)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4A: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4A Sample was from cerebral cortex.
Specimen 4B: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4B Sample was from midbrain.
Specimen 4C: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4C Sample was from cerebral cortex. Phosphorylated Smad3 expression level was reduced in the mutant.
Specimen 4D: embryonic day 14.5; Itgb8tm2Lfr/Itgb8+, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4D Sample was from midbrain. Phosphorylated Smad3 expression level was reduced in the mutant.
Specimen 4H: embryonic day 14.5 (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4H Sample was from cerebral cortex.
Specimen 4I: embryonic day 14.5 (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4I Sample was from midbrain.
Specimen 4J: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4J Sample was from cerebral cortex. Phosphorylated Smad3 expression level was reduced in the mutant.
Specimen 4K: embryonic day 14.5; Itgb8tm1Lfr/Itgb8tm1Lfr (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present 4K Sample was from midbrain. Phosphorylated Smad3 expression level was reduced in the mutant.
Specimen S6A,B: embryonic day 14.5; Tg(Nes-cre)1Kln/0 (more )
Note: Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Present S6A , S6B Sample was from the telencephalic region.
Specimen S6C,D: embryonic day 14.5; Itgb8tm2Lfr/Itgb8tm2Lfr, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Smad3; red - Pecam1 (assay MGI:7281812).
Structure Cell Type Level Pattern Image Note
TS22: blood vessel endothelial cell Weak S6C , S6D Sample was from the telencephalic region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/09/2024
MGI 6.24 		The Jackson Laboratory