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Gene Expression Data
Assay Details
Assay
Reference: J:310902 Hayashi S, et al., The nephric mesenchyme lineage of intermediate mesoderm is derived from Tbx6-expressing derivatives of neuro-mesodermal progenitors via BMP-dependent Osr1 function. Dev Biol. 2021 Oct;478:155-162
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7286068
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: anti-WT1 (C-19; sc-192)
Detection system: Secondary antibody
Results
Specimen 2K: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: metanephric mesenchyme Present 2K
Specimen 2L: embryonic day 10.5; Tbx6tm1Pa/Tbx6tm1Pa (more )
Structure Level Pattern Image Note
TS17: metanephric mesenchyme Absent 2L
Specimen 3A: embryonic day 9.5 (more )
Note: Forelimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).
Structure Level Pattern Image Note
TS15: mesonephros mesenchyme Present 3A
Specimen 3B: embryonic day 9.5; Tbx6tm1Pa/Tbx6tm1Pa (more )
Note: Forelimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).
Structure Level Pattern Image Note
TS15: mesonephros mesenchyme Present 3B Wt1-expressing cells and ectopic Sox2-expressing cells were intermingled.
Specimen 3E: embryonic day 9.5 (more )
Note: Hindlimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).
Structure Level Pattern Image Note
TS15: mesonephros mesenchyme Present 3E
Specimen 3F: embryonic day 9.5; Tbx6tm1Pa/Tbx6tm1Pa (more )
Note: Hindlimb level. Triple labeled: red - Wt1; white - Cdh1 (assay MGI:7286081); green - Sox2 (assay MGI:7286078).
Structure Level Pattern Image Note
TS15: mesonephros mesenchyme Present Regionally restricted 3F At the hindlimb level, Wt1 was not expressed, suggesting a defect in the posterior extension. Ectopic Sox2 was expressed in an open tube-like structure.
Specimen 4A: embryonic day 9.5 (more )
Note: Double labeled: red - Wt1; green - Cdh1 (assay MGI:7286082).
Structure Level Pattern Image Note
TS15: mesenchyme derived from splanchnopleure Present 4A
TS15: mesonephros mesenchyme Present 4A
Specimen 4B: embryonic day 9.5; Osr1em1Tkto/Osr1em1Tkto (more )
Note: Double labeled: red - Wt1; green - Cdh1 (assay MGI:7286082).
Structure Level Pattern Image Note
TS15: mesenchyme derived from splanchnopleure Present 4B
TS15: mesonephros mesenchyme Absent 4B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory