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Gene Expression Data
Assay Details
Assay
Reference: J:171508 Ikegami D, et al., Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development. 2011 Apr;138(8):1507-19
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7287487
Gene symbol: Akt1
Gene name: thymoma viral proto-oncogene 1
Antibody: anti-Phospho-Akt (Ser473) #4060
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 4G Sox9flox/+: embryonic day 13.5; Sox9tm2Crm/Sox9+ (more )
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Present Regionally restricted 4G Anti-pAkt Sox9flox/+ Expression was detected in the central part of primordial cartilage and not in the periphery.
Specimen 4G 11Enh-Cre; Sox9flox/flox: embryonic day 13.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS21: humerus cartilage condensation Absent 4G Anti-pAkt 11Enh-Cre; Sox9flox/flox
Specimen 4H Sox9flox/+: embryonic day 14.5; Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 4H Anti-pAkt Sox9flox/+ Expression was detected in flat proliferative chondrocytes.
Specimen 4H 11Prom-Cre; Sox9flox/flox: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Weak 4H Anti-pAkt 11Prom-Cre; Sox9flox/flox Expression was decreased in proliferative chondrocytes in the conditional mutant compared to control.
Specimen 5D Sox9flox/+; Ptenflox/+: embryonic day 14.5; Ptentm2Mak/Pten+, Sox9tm2Crm/Sox9+ (more )
Note: Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 5D Anti-phospho-Akt Sox9flox/+;Ptenflox/+ Expression was detected in proliferative chondrocytes.
Specimen 5D 11Prom-Cre; Sox9flox/flox; Pten+/+: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Weak Regionally restricted 5D Anti-phospho-Akt 11Prom-Cre;Sox9flox/flox;Pten+/+ Phosphorylation of Akt1 was reduced in proliferative chondrocytes of 11Prom-Cre; Sox9<flox/flox>; Pten<+/+> conditional mutant.
Specimen 5D 11Prom-Cre; Sox9flox/flox; Ptenflox/flox: embryonic day 14.5; Ptentm2Mak/Ptentm2Mak, Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant. Metaphyseal region of the humerus.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted 5D Anti-phospho-Akt 11Prom-Cre;Sox9flox/flox;Ptenflox/flox Phosphorylation levels of Akt1 were higher in the 11Prom-Cre; Sox9<flox/flox>; Pten<flox/flox> proliferative chondrocytes than in 11Prom-Cre; Sox9<flox/flox>; Pten<+/+>.
Specimen S5E Sox9flox/+: embryonic day 12.5; Sox9tm2Crm/Sox9+ (more )
Structure Level Pattern Image Note
TS20: humerus pre-cartilage condensation Absent S5E Anti-phospho-Akt Sox9flox/+
Specimen S5E 11Enh-Cre; Sox9flox/flox: embryonic day 12.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)1Ntsu/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: humerus pre-cartilage condensation Absent S5E Anti-phospho-Akt 11Enh-Cre;Sox9flox/flox
Specimen S6 Sox9flox/+; Ptenflox/+: embryonic day 14.5; Ptentm2Mak/Pten+, Sox9tm2Crm/Sox9+ (more )
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Anti-phospho-Akt Sox9flox/+;Ptenflox/+ Expression was detected in proliferative chondrocytes.
Specimen S6 11Prom-Cre; Sox9flox/flox; Pten+/+: embryonic day 14.5; Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Weak Regionally restricted S6_Anti-phospho-Akt 11Prom-Cre;Sox9flox/flox;Pten+/+ Expression was reduced in the Sox9-deleted proliferative chondrocytes in the 11Prom-Cre; Sox9<flox/flox>; Pten<+/+> conditional mutant.
Specimen S6 11Prom-Cre; Sox9flox/flox; Ptenflox/flox: embryonic day 14.5; Ptentm2Mak/Ptentm2Mak, Sox9tm2Crm/Sox9tm2Crm, Tg(Col11a2-cre)2Ntsu/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS22: humerus cartilage condensation chondrocyte Present Regionally restricted S6_Anti-phospho-Akt 11Prom-Cre;Sox9flox/flox;Ptenflox/flox Expression recovered in Sox9<flox/flox>; Pten<flox/flox> proliferative chondrocytes
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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