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Gene Expression Data
Assay Details
Assay
Reference: J:326587 Hurley S, et al., Distinct, dosage-sensitive requirements for the autism-associated factor CHD8 during cortical development. Mol Autism. 2021 Feb 24;12(1):16
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7316758
Gene symbol: Chd8
Gene name: chromodomain helicase DNA binding protein 8
Antibody: anti-CHD8 (A301-225A)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 5b Ctrl: embryonic day 10.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn (more )
Structure Level Pattern Image Note
TS17: telencephalon Present 5b Ctrl
Specimen 5b cKO: embryonic day 10.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn, Sox1tm1(cre)Take/Sox1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: telencephalon Absent 5b cKO
Specimen S9A,A',A'': embryonic day 12.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn (more )
Note: brain
Structure Level Pattern Image Note
TS20: pallium Present Widespread S9A Nuclear expression throughout pallium and subpallium.
TS20: neocortex Strong S9A , S9A' Higher levels of expression in dorsal cortex compared to ventral pallium.
TS20: ventral pallium Present S9A , S9A''
Specimen S9B,B',B'': embryonic day 11.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn (more )
Note: brain
Structure Level Pattern Image Note
TS19: telencephalon Present Widespread S9B'' , S9B , S9B' Nuclear expression throughout pallium and subpallium. Higher levels of expression in dorsal cortex compared to ventral pallium.
Specimen S9C,C',C'': embryonic day 10.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn (more )
Note: brain
Structure Level Pattern Image Note
TS17: telencephalon Present Widespread S9C'' , S9C , S9C' Nuclear expression throughout pallium and subpallium. Higher levels of expression in dorsal cortex compared to ventral pallium.
Specimen S9D,D': embryonic day 12.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn, Sox1tm1(cre)Take/Sox1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS20: brain Absent S9D' , S9D Widespread loss of expression in all brain structures.
Specimen S9E,E': embryonic day 11.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn, Sox1tm1(cre)Take/Sox1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: telencephalon Ambiguous S9E , S9E' Expression not reported for this specimen.
Specimen S9F,F': embryonic day 10.5; Chd8tm1.1Mabn/Chd8tm1.1Mabn, Sox1tm1(cre)Take/Sox1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: telencephalon Ambiguous S9F' , S9F Expression not reported for this specimen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
MGI 6.24 		The Jackson Laboratory