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Gene Expression Data
Assay Details
Assay
Reference: J:306393 Ofek S, et al., Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube. BMC Biol. 2021 Apr 23;19(1):84
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7338981
Gene symbol: Dll1
Gene name: delta like canonical Notch ligand 1
Antibody: DLL1 Antibody (AF5026)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 3M/N: embryonic day 9.75 (more )
Structure Level Pattern Image Note
TS16: neural tube lateral wall Present Widespread 3N , 3M
TS16: neural tube roof plate Absent 3M , 3N
Specimen 3O/P: embryonic day 9.75; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: neural tube lateral wall Present Widespread 3O , 3P Increased expression compared with the wild-type.
TS16: neural tube roof plate Present Regionally restricted 3P , 3O Expression shifted dorsally with significantly fewer negative cells in the dorsal midline region.
Specimen 3Q/R: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Widespread 3Q , 3R
TS17: neural tube roof plate Absent 3Q , 3R
Specimen 3S/T: embryonic day 10.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present 3T , 3S Increased expression compared with the wild-type.
TS17: neural tube roof plate Present Regionally restricted 3S , 3T Expression shifted dorsally with significantly fewer negative cells in the dorsal midline region.
Specimen 3U/V: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 3V , 3U
TS19: neural tube roof plate Absent 3V , 3U
Specimen 3W/X: embryonic day 11.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 3X , 3W Increased expression compared with the wild-type.
TS19: neural tube roof plate Present Widespread 3W , 3X Expression reached the dorsal midline with almost no negative cells remaining.
Specimen 3AA: embryonic day 11.5 (more )
Note: Double labeled: green - Dll1; red - Notch1 (assay MGI:7338980).
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 3AA The dorsal limit of expression coincided with that of Notch1.
TS19: neural tube roof plate Absent 3AA
Specimen 7M: embryonic day 11.5 (more )
Note: Double labeled: black - Dll1; blue - Lmx1a (assay MGI:7339057).
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 7M Complementary expression of Lmx1a and Dll1 was seen.
Specimen 7N: embryonic day 11.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: black - Dll1; blue - Lmx1a (assay MGI:7339057).
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 7N
TS19: neural tube roof plate Present 7N
Specimen S10G: embryonic day 10.5 (more )
Note: Double labeled: green - Dll1; red - anti-EdU antibody.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present S10G
TS17: neural tube roof plate Absent S10G
Specimen S10H: embryonic day 10.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant. Double labeled: green - Dll1; red - anti-EdU antibody.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present S10H Increased expression compared with the wild-type.
TS17: neural tube roof plate Present Regionally restricted S10H Expression shifted dorsally with significantly fewer negative cells in the dorsal midline region.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/15/2024
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