About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:226322 Roux M, et al., Hoxb1 regulates proliferation and differentiation of second heart field progenitors in pharyngeal mesoderm and genetically interacts with Hoxa1 during cardiac outflow tract development. Dev Biol. 2015 Oct 15;406(2):247-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7345660
Gene symbol: Mapk1
Gene name: mitogen-activated protein kinase 1
Antibody: Anti-pERK
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody used was not detailed; both family members have been annotated.
Results
Specimen 2F: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: branchial arch endoderm Ambiguous 2F
TS15: branchial arch mesenchyme Ambiguous 2F
Specimen 2G: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Structure Level Pattern Image Note
TS15: branchial arch endoderm Present 2G Increased number of positive cells in the ventral pharyngeal region.
TS15: branchial arch mesenchyme Present 2G Increased number of positive cells in the ventral pharyngeal region and in the dorsal pericardial wall.
Specimen 4K: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1+ (more )
Structure Level Pattern Image Note
TS15: branchial arch mesenchyme Present Scattered 4K
TS15: foregut epithelium Present Scattered 4K
Specimen 4L: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1tm1Ipc (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 4L Similar as in the wild-type (quantified in 6E).
TS15: branchial arch mesenchyme Present 4L Similar as in the wild-type (quantified in 6F).
Specimen 6A: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1+, Hoxb1tm5Mrc/Hoxb1+ (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 6A Similar as in the wild-type (quantified in 6E).
TS15: branchial arch mesenchyme Present 6A Increased expression compared with the wild-type (quantified in 6F).
Specimen 6B: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1tm1Ipc, Hoxb1tm5Mrc/Hoxb1+ (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 6B Increased expression compared with the wild-type (quantified in 6E).
TS15: branchial arch mesenchyme Present 6B Similar as in the wild-type (quantified in 6F).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
06/12/2024
MGI 6.13 		The Jackson Laboratory