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Gene Expression Data
Assay Details
Assay
Reference: J:226322 Roux M, et al., Hoxb1 regulates proliferation and differentiation of second heart field progenitors in pharyngeal mesoderm and genetically interacts with Hoxa1 during cardiac outflow tract development. Dev Biol. 2015 Oct 15;406(2):247-58
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7345664
Gene symbol: Smad1
Gene name: SMAD family member 1
Antibody: anti-phospho-Smad1/5/8
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The antibody used recognizes the phosphorylated form of the protein. The specificity of the antibody was specified as Smad1 in the paper.
Results
Specimen 3C: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: branchial arch mesenchyme Present 3C
TS15: foregut epithelium Present 3C
Specimen 3D: embryonic day 9.5; Hoxb1tm5Mrc/Hoxb1tm5Mrc (more )
Structure Level Pattern Image Note
TS15: branchial arch mesenchyme Present 3D Similar as in the wild-type.
TS15: foregut epithelium Present 3D Increased expression in the ventral endoderm (quantified in 3E).
Specimen 4M: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: branchial arch mesenchyme Present 4M
TS15: foregut epithelium Present 4M
Specimen 4N: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1tm1Ipc (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 4N Similar as in the wild-type (quantified in 6G).
TS15: branchial arch mesenchyme Present 4N Similar as in the wild-type (quantified in 6H).
Specimen 6C: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1+, Hoxb1tm5Mrc/Hoxb1+ (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 6C Similar as in the wild-type (quantified in 6G).
TS15: branchial arch mesenchyme Present 6C Similar as in the wild-type (quantified in 6H).
Specimen 6D: embryonic day 9.5; Hoxa1tm1Ipc/Hoxa1tm1Ipc, Hoxb1tm5Mrc/Hoxb1+ (more )
Structure Level Pattern Image Note
TS15: foregut epithelium Present 6D Similar as in the wild-type (quantified in 6G).
TS15: branchial arch mesenchyme Present 6D Similar as in the wild-type (quantified in 6H).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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