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Gene Expression Data
Assay Details
Assay
Reference: J:240523 Yang Z, et al., Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016 Oct 01;418(1):66-74
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7346135
Gene symbol: Podxl2
Gene name: podocalyxin-like 2
Antibody: Mouse Endoglycan/PODXL2 Antibody (AF3534)
Detection system: Secondary antibody
Results
Specimen 1B: postnatal day 0 (more )
Note: Cap mesenchyme stage. Triple labeled: red - Podxl2; blue - Prkci (assay MGI:7346141); green - Ncam1 (assay MGI:7346139).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present Punctate 1B middle , 1B right upper , 1B left Expressed in numerous intracellular puncta that appeared vesicular in nature, and not polarized.
Specimen 1C: postnatal day 0 (more )
Note: Pretubular aggregate stage. Double labeled: red - Podxl2; green - Ncam1 (assay MGI:7346139).
Structure Level Pattern Image Note
TS27: pretubular aggregate Present Clusters 1C left , 1C right upper , 1C right merge Expressed in the puncta, which appeared to cluster and form higher-order structures.
Specimen 1D: postnatal day 0 (more )
Note: Renal vesicle stage. Double labeled: red - Podxl2; green - Ncam1 (assay MGI:7346139). Cyan - nuclear staining.
Structure Level Pattern Image Note
TS27: renal vesicle Present Clusters 1D right upper , 1D left During lumen formation, positive puncta relocalized to the apical cell surface.
Specimen 2A: postnatal day 0 (more )
Note: Triple labeled: red - Podxl2; green - Rab11a (assay MGI:7346151) and Rab11b (assay MGI:7346428); blue - Ncam1 (assay MGI:7346140).
Structure Level Pattern Image Note
TS27: cap mesenchyme Present Punctate 2A left , 2A right lower Coexpressed with Rab11a/b, a known marker of recycling endosomes.
Specimen 2B: postnatal day 0 (more )
Note: Triple labeled: red - Podxl2; green - Rab11a (assay MGI:7346151) and Rab11b (assay MGI:7346428); blue - Ncam1 (assay MGI:7346140).
Structure Level Pattern Image Note
TS27: renal vesicle Present Regionally restricted 2B right lower , 2B left Positive puncta relocalized to the apical cell surface. The amount of colocalization with Rab11a/b decreased after lumen initiation.
Specimen 3A: postnatal day 0 (more )
Note: Triple labeled: red - Podxl2; blue - Ncam1 (assay MGI:7346140); green - Ezr (assay MGI:7346165).
Structure Level Pattern Image Note
TS27: renal vesicle Present Regionally restricted 3A right , 3A left Coexpressed with Ezr in the intraluminal space.
Specimen 3B: postnatal day 0 (more )
Note: Triple labeled: red - Podxl2; blue - Ncam1 (assay MGI:7346140); green - Ezr (assay MGI:7346165).
Structure Level Pattern Image Note
TS27: renal vesicle Present 3B left , 3B middle Coexpressed with Ezr in the intraluminal space.
Specimen 5A CTL: embryonic day 14.5; Podxltm1Parl/Podxltm1Parl, Podxl2tm1c(EUCOMM)Wtsi/Podxl2tm1c(EUCOMM)Wtsi (more )
Note: Triple labeled: red - Podxl2; blue - Ncam1 (assay MGI:7346140); green - Pard6b (assay MGI:7346199).
Structure Level Pattern Image Note
TS22: renal vesicle Present Regionally restricted 5A CTL right upper , 5A CTL left Expressed in the apical, luminal surfaces.
Specimen 5A cKO: embryonic day 14.5; Podxltm1Parl/Podxltm1Parl, Podxl2tm1c(EUCOMM)Wtsi/Podxl2tm1c(EUCOMM)Wtsi, Tg(Pax3-cre)1Joe/0 (more )
Note: Conditional mutant. Triple labeled: red - Podxl2; blue - Ncam1 (assay MGI:7346140); green - Pard6b (assay MGI:7346199).
Structure Level Pattern Image Note
TS22: renal vesicle Absent 5A CKO right upper , 5A CKO left
Specimen not shown: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: renal interstitium Present renal stroma
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory