Probe:	Mm-Hoxd11 (580621)
Probe preparation:	Antisense
Visualized with:	Fluorescence
Assay notes:	RNAscope was performed using the RED2.5 detection system.

Structure	Level	Pattern	Image	Note
TS23: forelimb	Present	Regionally restricted	7B	Expression was more specific and restricted to the posterior regions of the limb and extended more prominently into the proximal zeugopod to include the olecranon. Expression was excluded from the developing distal growth plates.
TS23: zeugopod	Present	Regionally restricted	7B	Expression was more specific and restricted to the posterior regions of the limb and extended more prominently into the proximal zeugopod to include the olecranon. Expression was excluded from the developing distal growth plates.
TS23: ulna	Present	Regionally restricted	7B	Expression extended into the olecranon.

Structure	Level	Pattern	Image	Note
TS27: wrist	Present	Regionally restricted	7E	Expression was restricted to posterior wrist elements.

Structure	Level	Pattern	Image	Note
TS27: elbow	Weak		7G

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Absent		7I	No expression in the articular cartilage of the adult wrist.

Structure	Level	Pattern	Image	Note
TS28: synovium	Weak	Regionally restricted	7K	Low expression in synovial lining of the wrist.

Structure	Level	Pattern	Image	Note
TS23: hindlimb	Present	Regionally restricted	8C	Expression was largely restricted to the posterior region of zeugopod and display much lower expression in zeugopod joints.
TS23: zeugopod	Present	Regionally restricted	8C	Expression was largely restricted to the posterior region of zeugopod and display much lower expression in zeugopod joints.
TS23: ankle	Present	Regionally restricted	8C	Expression was detected in the developing achilles tendon.

Structure	Level	Pattern	Image	Note
TS27: ankle	Weak		8F
TS27: tendo calcaneus	Weak		8F

Structure	Level	Pattern	Image	Note
TS27: knee joint	Absent		8G Hoxd11

Structure	Level	Pattern	Image	Note
TS27: knee joint	Absent		8H Hoxd11

Structure	Level	Pattern	Image	Note
TS27: knee joint	Absent		8I Hoxd11

Structure	Level	Pattern	Image	Note
TS27: knee joint	Absent		8J Hoxd11

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Ambiguous		8K Hoxd11

Structure	Level	Pattern	Image	Note
TS28: synovium	Ambiguous		8L Hoxd11

Structure	Level	Pattern	Image	Note
TS28: knee joint	Ambiguous		8M Hoxd11

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Regionally restricted	8N Hoxd11	Expression was detected in the knee ligament.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present	Regionally restricted	8O Hoxd11	Expression was detected in the tibia bone marrow.

Structure	Level	Pattern	Image	Note
TS23: shoulder	Absent		S5C

Structure	Level	Pattern	Image	Note
TS23: hip	Absent		S5F

Structure	Level	Pattern	Image	Note
TS23: sacral vertebral cartilage condensation	Present		S5I