Gene Expression Data
Assay Details
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Assay
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Reference:
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J:314181
Holzman MA, et al., HOXA5 Participates in Brown Adipose Tissue and Epaxial Skeletal Muscle Patterning and in Brown Adipocyte Differentiation. Front Cell Dev Biol. 2021;9:632303
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7384941
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Gene symbol:
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Pparg
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Gene name:
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peroxisome proliferator activated receptor gamma
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Results
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Specimen
1I/J:
postnatal month 3; Tg(Hoxa5-cre)447BLjea/0
(more  )
Note: Double labeled: red - Pparg; green - Cre reporter.
Specimen
1I/J:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Tg(Hoxa5-cre)447BLjea/0
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Age:
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postnatal month 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pparg; green - Cre reporter.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: brown fat |
brown adipocyte |
Present |
Regionally restricted |
1J
,
1I
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Nuclear expression in adipocytes.
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Specimen
5A:
embryonic day 18.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
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5A
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Specimen
5B:
embryonic day 18.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
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5B
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Specimen
5F:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5F
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Adipocyte density appeared reduced compared to controls.
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Specimen
5G:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: brown fat |
brown adipocyte |
Present |
Regionally restricted |
5G
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Adipocyte density appeared reduced compared to controls.
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Specimen
S3A/B/C:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Hoxa5tm1.1Ljea/Hoxa5+
Meox1tm1(cre)Jpa/Meox1+
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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Specimen
S3E/F/G:
(close )
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Genetic Background:
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involves: 129/Sv * C57BL/6J * SJL
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Mutant Allele(s):
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Hoxa5tm1.1Ljea/Hoxa5tm1.1Ljea
Meox1tm1(cre)Jpa/Meox1+
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Conditional mutant.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS27: brown fat |
brown adipocyte |
Present |
Regionally restricted |
S3G
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S3E
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S3F
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Smaller BAT area and reduced adipocyte density compared to control.
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Specimen
8A:
embryonic day 14.5; Hoxa5tm1Rob/Hoxa5 +
(more )
Note: Double labeled: green - Pparg; red - Myh4 (assay MGI:7384937).
Specimen
8A:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5+
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pparg; red - Myh4 (assay MGI:7384937).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brown fat |
brown adipocyte |
Present |
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8A
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Specimen
8B:
embryonic day 14.5
(more )
Specimen
8B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brown fat |
brown adipocyte |
Present |
Regionally restricted |
8B
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Smaller BAT area and reduced adipocyte density compared to control.
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Specimen
8C:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pparg; red - Myh4 (assay MGI:7384937).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brown fat |
brown adipocyte |
Present |
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8C
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Specimen
8D:
(close )
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Genetic Background:
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involves: 129S/SvEv
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Mutant Allele(s):
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Hoxa5tm1Rob/Hoxa5tm1Rob
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS22: brown fat |
brown adipocyte |
Present |
Regionally restricted |
8D
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Smaller BAT area and reduced adipocyte density compared to control.
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Analysis Tools