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Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388340
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 6A: embryonic day 13.5 (more )
Note: Double labeled: blue - Acta2; red - BrdU staining.
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present 6A
Specimen 6B: embryonic day 13.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: blue - Acta2; red - BrdU staining.
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present 6B Cell proliferation in the mesenchyme was similar as in the control.
Specimen 6C: embryonic day 13.5 (more )
Note: Double labeled: blue - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present 6C
Specimen 6D: embryonic day 13.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: blue - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme smooth muscle cell of trachea Present 6D Cell proliferation in the mesenchyme was similar as in the control.
Specimen 8G: embryonic day 16.5 (more )
Note: Triple labeled: blue - Acta2; green - Nkx2-1 (assay MGI:7388342); red - Ctnnb1 (assay MGI:7388343).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme smooth muscle cell of trachea Present 8G
Specimen 8H: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: blue - Acta2; green - Nkx2-1 (assay MGI:7388342); red - Ctnnb1 (assay MGI:7388343).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme smooth muscle cell of trachea Present 8H
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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