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Gene Expression Data
Assay Details
Assay
Reference: J:331142 Han P, et al., Cbln1 regulates axon growth and guidance in multiple neural regions. PLoS Biol. 2022 Nov;20(11):e3001853
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388453
Gene symbol: Cbln1
Gene name: cerebellin 1 precursor protein
Antibody: anti-Cbln1
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-Cbln1 MGI:7388431 may have been used in this assay.
Results
Specimen 1B Cbln1 (left): embryonic day 10.5 (more )
Note: Double labeled: green - Cbln1; red - Lhx2 (assay MGI:7388454).
Structure Cell Type Level Pattern Image Note
TS17: future spinal cord commissural neuron Present Regionally restricted 1B Cbln1 (left) , 1B (merged left) Co-expression with Lhx2 in dI1 dorsal commissural neurons.
Specimen 1B Cbln1 (right): embryonic day 10.5 (more )
Note: Double labeled: green - Cbln1; red - Alcam (assay MGI:7388455).
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 1B (merged right) , 1B Cbln1 (right) Co-expression with Alcam in floor plate.
Specimen 1C Cbln1 (left): embryonic day 11.5 (more )
Note: Double labeled: green - Cbln1; red - Lhx2 (assay MGI:7388454).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted 1C (merged left) , 1C' , 1C Cbln1 (left) Co-expression iwith Lhx2 in dI1 dorsal commissural neurons.
Specimen 1C Cbln1 (right): embryonic day 11.5 (more )
Note: Double labeled: green - Cbln1; red - Alcam (assay MGI:7388455).
Structure Level Pattern Image Note
TS19: neural tube floor plate Present 1C Cbln1 (right) , 1C (merged right) Co-expression with Alcam in floor plate.
Specimen 1D Cbln1 (upper): embryonic day 12.5 (more )
Note: Double labeled: green - Cbln1; red - Lhx2 (assay MGI:7388454).
Structure Cell Type Level Pattern Image Note
TS20: spinal cord commissural neuron Present Regionally restricted 1D (merged upper) , 1D' , 1D Cbln1 (upper) Co-expression iwith Lhx2 in dI1 dorsal commissural neurons.
Specimen 1D Cbln1 (lower): embryonic day 12.5 (more )
Note: Double labeled: green - Cbln1; red - Alcam (assay MGI:7388455).
Structure Level Pattern Image Note
TS20: spinal cord floor plate Present 1D (merged lower) , 1D Cbln1 (lower) Co-expression with Alcam in floor plate.
Specimen S1D Cbln1: embryonic day 11.0 (more )
Note: Double labeled: green - Cbln1; red - Lhx1 (assay MGI:7388456) and Lhx5 (assay MGI:7388457).
Structure Cell Type Level Pattern Image Note
TS18: future spinal cord commissural neuron Present Regionally restricted S1D (merged) , S1D Cbln1 Expression in dorsal commissural neurons. Expression in dI2 neurons marked by Lim1/2 expression
Specimen S1E Cbln1: embryonic day 11.0 (more )
Note: Triple labeled: green - Cbln1; red - Pou4f1 (assay MGI:7388458); blue - Isl1 (assay MGI:7388460) and Isl2 (assay MGI:7388461).
Structure Cell Type Level Pattern Image Note
TS18: future spinal cord commissural neuron Present Regionally restricted S1E (merged) , S1E Cbln1 Expression in dorsal commissural neurons. Expression in dI3 neurons co-expressing Isl1/2 and Brn3a (Pou4f1) and dI4 neurons co-expressing Brn3a.
Specimen S1F Gli2+/- Cbln1: embryonic day 11.5; Gli2tm1Alj/Gli2+ (more )
Note: Double labeled: green - Cbln1; red - Alcam (assay MGI:7388455).
Structure Level Pattern Image Note
TS19: neural tube floor plate Present S1F Gli2+/- (merged lower) , S1F Gli2+/- (merged upper) , S1F Gli2+/- Cbln1 (upper) , S1F Gli2+/- Cbln1 (lower)
Specimen S1F Gli2-/- Cbln1: embryonic day 11.5; Gli2tm1Alj/Gli2tm1Alj (more )
Note: Double labeled: green - Cbln1; red - Alcam (assay MGI:7388455).
Structure Level Pattern Image Note
TS19: neural tube floor plate Absent S1F Gli2-/- Cbln1 (upper) , S1F Gli2-/- (merged upper) , S1F Gli2-/- Cbln1 (lower) , S1F Gli2-/- (merged lower)
Specimen 6B,B': postnatal day 8 (more )
Structure Cell Type Level Pattern Image Note
TS28: cerebellum molecular layer cerebellar granule cell Strong Regionally restricted 6B (merged) , 6B' (merged) , 6B Cbln1 , 6B' Cbln1 High level of expression in molecular layer, secreted by granule cell axons.
Specimen 7C Anterior Cbln1: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: diencephalon floor plate Present Regionally restricted 7C Merged Anterior , 7C Cbln1 Anterior Expression in floor of third ventricle, adjacent to optic chiasm.
Specimen 7C (middle) Cbln1: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: diencephalon floor plate Present Regionally restricted 7C Merged (center) , 7C Cbln1 (center) Expression in floor of third ventricle, adjacent to optic chiasm.
Specimen 7C Posterior Cbln1: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: diencephalon floor plate Present Regionally restricted 7C Cbln1 Posterior , 7C Merged Posterior Expression in floor of third ventricle, adjacent to optic chiasm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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