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Gene Expression Data
Assay Details
Assay
Reference: J:331142 Han P, et al., Cbln1 regulates axon growth and guidance in multiple neural regions. PLoS Biol. 2022 Nov;20(11):e3001853
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388458
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Antibody: anti-Brn3a
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S1E Brn3a: embryonic day 11.0 (more )
Note: Triple labeled: red - Pou4f1; green - Cbln1 (assay MGI:7388453); blue - Isl1 (assay MGI:7388460) and Isl2 (assay MGI:7388461).
Structure Cell Type Level Pattern Image Note
TS18: future spinal cord commissural neuron Present Regionally restricted S1E (merged) , S1E Brn3a Expression in dI3 and dI4 dorsal commissural neurons.
Specimen S2E Cbln1fl/fl Brn3a: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj (more )
Note: Double labeled: red - Pou4f1; green - Isl1 (assay MGI:7388460) and Isl2 (assay MGI:7388461).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Cbln1fl/fl (merged) , S2E Cbln1fl/fl Brn3a Expression in dI3 and dI4 dorsal commissural neurons.
Specimen S2E Wnt1-Cre Brn3a: embryonic day 11.5; Cbln1tm1.1Sjj/Cbln1tm1.1Sjj, H2az2Tg(Wnt1-cre)11Rth/0 (more )
Note: Conditional mutant. Double labeled: red - Pou4f1; green - Isl1 (assay MGI:7388460) and Isl2 (assay MGI:7388461).
Structure Cell Type Level Pattern Image Note
TS19: future spinal cord commissural neuron Present Regionally restricted S2E Wnt1-Cre (merged) , S2E Wnt1-Cre Brn3a Patterning not affected for dI3 or dI4 neurons.
Specimen 7H Brn3a: embryonic day 13.0 (more )
Note: Double labeled: green - Pou4f1; red - Nrxn2 (assay MGI:7388468).
Structure Cell Type Level Pattern Image Note
TS21: retina retinal ganglion cell Present 7H Merged , 7H Brn3a
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory