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Gene Expression Data
Assay Details
Assay
Reference: J:154510 Gebauer JM, et al., Mouse AMACO, a kidney and skin basement membrane associated molecule that mediates RGD-dependent cell attachment. Matrix Biol. 2009 Oct;28(8):456-62
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7414227
Gene symbol: Vwa2
Gene name: von Willebrand factor A domain containing 2
Antibody: anti-AMACO-P3
Detection system: Secondary antibody coupled to Alexa Fluor 546
Results
Specimen 1A: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Lama1 (assay MGI:7414232).
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 1A Expression was localized at the basement membrane of the branching ureter bud mainly at the branching points.
TS27: collecting duct Present Regionally restricted 1A Expression was localized at the basement membrane of the branching ureter bud mainly at the branching points.
Specimen 1B: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Lama1 (assay MGI:7414232).
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 1B Expression was detected in the basement membrane of S-shaped bodies. Signal partially colocalized with Lama1 labeling.
TS27: s-shaped body Present Regionally restricted 1B Expression was detected in the basement membrane of S-shaped bodies. Signal partially colocalized with Lama1 labeling.
Specimen 1C: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Pecam1 (assay MGI:7414234).
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 1C Expression was detected in the basement membrane of S-shaped bodies. Signal did not colocalize with Pecam1 labeling in blood vessels.
TS27: s-shaped body Present Regionally restricted 1C Expression was detected in the basement membrane of S-shaped bodies. Signal did not colocalize with Pecam1 labeling in blood vessels.
Specimen 1D: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Lama1 (assay MGI:7414232).
Structure Level Pattern Image Note
TS27: macula densa Present 1D
TS27: glomerular basement membrane of mature renal corpuscle Absent 1D
Specimen 1E: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Lama5 (assay MGI:7414233).
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 1E Expression in basement membranes of tubular structures in the medulla colocalizes with Lama5 labeling.
TS27: renal medulla Present Regionally restricted 1E Expression in basement membranes of tubular structures in the medulla colocalizes with Lama5 labeling.
TS27: renal tubule Present Regionally restricted 1E Expression in basement membranes of tubular structures in the medulla colocalizes with Lama5 labeling.
Specimen 1F: postnatal newborn (more )
Note: Double labeled: red - Vwa2; green - Lama1 (assay MGI:7414232).
Structure Level Pattern Image Note
TS27: basement membrane Present Regionally restricted 1F Expression in basement membranes of tubular structures did not colocalize with Lama1 labeling.
TS27: renal tubule Present Regionally restricted 1F Expression in basement membranes of tubular structures did not colocalize with Lama1 labeling.
Specimen 3C: postnatal adult (more )
Structure Level Pattern Image Note
TS28: basement membrane Present 3C
TS28: epidermal-dermal junction Present 3C
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
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