Gene Expression Data
Assay Details
Assay
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Reference:
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J:332131
Perera SN, et al., Insights into olfactory ensheathing cell development from a laser-microdissection and transcriptome-profiling approach. Glia. 2020 Dec;68(12):2550-2584
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7424906
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Gene symbol:
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Ptn
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Gene name:
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pleiotrophin
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Probe:
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Ptn cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2 - Ptn:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
2 - Ptn:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S6 - E11.5:
embryonic day 11.5
(more )
Note: Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S6 - E11.5:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Parasagittal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Specimen
S6 - E13 - I1:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S6 - E13 - I1:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
|
Specimen
S6 - E13 - I2:
embryonic day 13.0
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
Specimen
S6 - E13 - I2:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Schwann cells and Tubb3 (magenta) to identify axons.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS21: olfactory nerve |
olfactory ensheathing cell |
Present |
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S6J2
,
S6I2
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The mucosal OECs show expression.
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TS21: head mesenchyme |
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Strong |
Regionally restricted |
S6I2
,
S6J2
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Strong expression is seen in the olfactory mucosal mesenchyme. No expression is seen in the mesenchyme surrounding the olfactory nerve layer.
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TS21: olfactory epithelium |
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Present |
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S6J2
,
S6I2
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Expressing cells are likely to include olfactory receptor neurons.
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Specimen
12g:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12g:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Specimen
12h:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12h:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
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Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: main olfactory bulb olfactory nerve layer |
olfactory ensheathing cell |
Present |
Non-Uniform |
12h1
,
12h
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The inner ONL-OECs show stronger expression than the outer ones. Expression levels in the mutant are higher than those seen in wild type.
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Specimen
12i:
embryonic day 16.5
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12i:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: olfactory nerve |
olfactory ensheathing cell |
Present |
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12i
,
12i1
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The mucosal OECs show expression.
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Specimen
12j:
embryonic day 16.5; Ptprz1tm1Mno/ Ptprz1tm1Mno
(more )
Note: Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
Specimen
12j:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptprz1tm1Mno/Ptprz1tm1Mno
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Coronal section. Immunostained with Sox10 (green) to label olfactory entheathing cells (OECs) and Tubb3 (magenta) to identify axons.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: olfactory nerve |
olfactory ensheathing cell |
Present |
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12j1
,
12j
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The mucosal OECs show expression. Expression levels in the mutant are higher than those seen in wild type.
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