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Gene Expression Data
Assay Details
Assay
Reference: J:332497 Tang X, et al., EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly. iScience. 2023 Jan 20;26(1):105667
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427876
Gene symbol: Emc4
Gene name: ER membrane protein complex subunit 4
Antibody: anti-EMC4 (ab184544)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Anti-EMC4 (ab236141) MGI:7427871 may have been used in this assay.
Results
Specimen 2A control: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3tm1c(EUCOMM)Wtsi (more )
Note: Triple labeled: red - Emc4; purple - Foxa2 (assay MGI:7427877); green - phospho-histone H3.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present 2A control (lower) , 2A control (upper)
Specimen 2A Emc3 cKO: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3tm1c(EUCOMM)Wtsi, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: red - Emc4; purple - Foxa2 (assay MGI:7427877); green - phospho-histone H3.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present 2A Emc3 cKO inset (lower) , 2A Emc3 cKO inset (upper) , 2A Emc3 cKO (lower) , 2A Emc3 cKO (upper)
Specimen 2B control: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3tm1c(EUCOMM)Wtsi (more )
Note: Triple labeled: red - Emc4; purple - Foxa2 (assay MGI:7427877); green - BrdU.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present 2B control (upper) , 2B control (lower)
Specimen 2B Emc3 cKO: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3tm1c(EUCOMM)Wtsi, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: red - Emc4; purple - Foxa2 (assay MGI:7427877); green - BrdU.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present 2B Emc3 cKO inset , 2B Emc3 cKO (lower) , 2B Emc3 cKO (upper)
Specimen S4A: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3+, Tg(CAG-cat,-EGFP)1Rbns/0, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Emc4; green - Cre-reporter.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present S4A EMC3/EGFP , S4A EMC4 , S4A (merge)
Specimen S4B: embryonic day 16.5; Emc3tm1c(EUCOMM)Wtsi/Emc3tm1c(EUCOMM)Wtsi, Tg(CAG-cat,-EGFP)1Rbns/0, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Double labeled: red - Emc4; green - Cre-reporter.
Structure Level Pattern Image Note
TS24: lung mesenchyme Present S4B EMC4/EGFP , S4B EMC4 , S4B (merge)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory