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Gene Expression Data
Assay Details
Assay
Reference: J:209131 Saba R, et al., CYP26B1 promotes male germ cell differentiation by suppressing STRA8-dependent meiotic and STRA8-independent mitotic pathways. Dev Biol. 2014 May 15;389(2):173-81
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7428651
Gene symbol: Gcna
Gene name: germ cell nuclear acidic protein
Antibody: TRA98 (ab82527)
Detection system: Secondary antibody coupled to Alexa Fluor 633
Results
Specimen 1B: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1B
Specimen 1C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1C
Specimen 1D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1D
Specimen 1E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1E
Specimen 1F: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1F
Specimen 1G: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 1G
Specimen 2A: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2A
Specimen 2B: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2B
Specimen 2C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2C
Specimen 2D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2D
Specimen 2E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2E
Specimen 2F: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 2F
Specimen 3A: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - BrdU.
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3A No germ cells showed BrdU incorporation.
Specimen 3B: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - BrdU.
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3B BrdU incorporation was clearly observed.
Specimen 3C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - BrdU.
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3C BrdU incorporation was clearly observed.
Specimen 3D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Mki67 (assay MGI:7428675).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3D
Specimen 3E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Mki67 (assay MGI:7428675).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3E
Specimen 3F: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Mki67 (assay MGI:7428675).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 3F
Specimen 3G: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Mki67 (assay MGI:7428675).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3G
Specimen 3H: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Mki67 (assay MGI:7428675).
Structure Level Pattern Image Note
TS26: germ cell of testis Present 3H
Specimen 4C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4C
Specimen 4D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4D
Specimen 4E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS23: germ cell of testis Present 4E
Specimen 5D: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 5D
Specimen 5E: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 5E
Specimen 5F: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Nanos2tm1Ysa/Nanos2tm1Ysa, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Kit (assay MGI:7428692).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 5F
Specimen S1A: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1A
Specimen S1B: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1B
Specimen S1C: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1C
Specimen S1D: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1D
Specimen S1E: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1E
Specimen S1F: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Sycp3 (assay MGI:7428650).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1F
Specimen S1G: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1G
Specimen S1H: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1H
Specimen S1I: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S1I
Specimen S1J: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1J
Specimen S1K: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1K
Specimen S1L: embryonic day 16.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - H2ax (assay MGI:7428652).
Structure Level Pattern Image Note
TS24: germ cell of testis Present S1L
Specimen S2A: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2A
Specimen S2B: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2B
Specimen S2C: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2C
Specimen S2D: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S2D
Specimen S2E: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Nanos2 (assay MGI:7428653).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S2E
Specimen S2F: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2F
Specimen S2G: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2G
Specimen S2H: embryonic day 14.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS22: germ cell of testis Present S2H
Specimen S2I: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S2I
Specimen S2J: embryonic day 18.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Dnmt3l (assay MGI:7428654).
Structure Level Pattern Image Note
TS26: germ cell of testis Present S2J
Specimen S6C: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1+, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Zbtb16 (assay MGI:7428809).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S6C
Specimen S6D: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8+ (more )
Note: Double labeled: magenta - Gcna; green - Zbtb16 (assay MGI:7428809).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S6D
Specimen S6E: embryonic day 15.5; Cyp26b1tm1Hmd/Cyp26b1tm1Hmd, Stra8tm1Ysa/Stra8tm1Ysa (more )
Note: Double labeled: magenta - Gcna; green - Zbtb16 (assay MGI:7428809).
Structure Level Pattern Image Note
TS23: germ cell of testis Present S6E
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MGI 6.24 		The Jackson Laboratory